纳米铜对肾细胞的氧化损伤作用

目的研究氧化应激在纳米铜引起肾细胞毒性中的作用。方法通过检测细胞内活性氧生成、金属硫蛋白表达、谷胱甘肽和丙二醛含量来了解纳米铜对肾细胞的氧化损伤作用。结果纳米铜可以剂量依赖性方式诱导细胞内ROS含量升高。20 mg.L-1纳米铜与细胞作用24 h,细胞内MT表达均明显增强。将0～40 mg.L-1纳米铜与HK-2细胞作用24 h,可引起细胞内GSH,培养上清中的MDA水平均呈剂量依赖性升高,而细胞内MDA、CuZn-SOD水平升高不明显。结论纳米铜在一定剂量和暴露时间条件下破坏了肾细胞内活性氧与抗氧化物质的动态平衡,引起氧化应激,这是纳米铜引起毒性损伤的机制之一。

Oxidative damage on HK-2 cells induced by nano-copper particles

Aim To study the oxidative damage on HK-2 cells induced by nano-copper particles.Methods The contents of ROS,MT,GSH,MDA and CuZn-SOD were measured respectively by different methods.Results The content of ROS in HK-2 cells increased dose-dependently when nano-copper particles exposed.After exposed 24 hours,the MT in HK-2 cells was induced.When 0～40 mg·L-1 nano-copper particles were exposed to HK-2 cells for 24 h,the content of MDA in cell and CuZn-SOD in cultured medium increased.Conclusion Nano-copper particles can induce oxidative stress,resulting from the imbalance of active oxygen and antioxygen.