| 黄芪有效成分对缺氧缺糖/复氧复糖大鼠海马神经元cyt-c、CcO表达的影响 |
| |
| 引用本文： | 焦俊霞,高维娟,钱涛,朱炎杰,董雅洁.黄芪有效成分对缺氧缺糖/复氧复糖大鼠海马神经元cyt-c、CcO表达的影响[J].中国药理学通报,2011,27(2):211-215. |
| |
| 作者姓名： | 焦俊霞 高维娟 钱涛 朱炎杰 董雅洁 |
| |
| 作者单位： | 承德医学院病理生理学教研室,河北,承德,067000 |
| |
| 基金项目： | 教育部新世纪优秀人才支持计划项目,河北省自然科学基金重点项目 |
| |
| 摘 要： | 目的观察黄芪有效成分对缺氧缺糖/复氧复糖大鼠海马神经元cyt-c及CcO表达的影响,探讨黄芪有效成分防治海马神经元凋亡可能的作用机制。方法取培养8 d的大鼠海马神经元,进行缺氧缺糖0.5 h,再分别复氧复糖0、0.5、2、6、24、72和120 h。实验分4组:正常对照组、模型组(缺氧缺糖/复氧复糖组)、黄芪注射液溶剂对照组和黄芪注射液组。分别采用细胞免疫化学法、Western blot法检测海马神经元cyt-c蛋白的表达、RT-PCR法检测CcO mRNA的表达。结果模型组在各个时间点cyt-c蛋白和CcO mRNA表达均较正常对照组增加(P<0.05);与模型组相比,黄芪有效成分组在各个时间点cyt-c蛋白和CcO mRNA表达均降低(P<0.05),而黄芪注射液溶剂对照组则无变化(P>0.05)。结论黄芪注射液可抑制缺氧缺糖/复氧复糖大鼠海马神经元cyt-c、CcO的表达,从而抑制缺氧缺糖/复氧复糖后大鼠海马神经元的凋亡。
|
| 关 键 词： | 细胞凋亡 缺氧缺糖/复氧复糖 黄芪注射液 cyt-c CcO 海马神经元 |
Effective compositions of astragalus injection affects the expression of cyt-c and CcO following oxygen-glucose deprivation and reintroduction in rat hippocampal neurons |
| |
| JIAO Jun-xia,GAO Wei-juan,QIAN Tao,ZHU Yan-jie,DONG Ya-jie.Effective compositions of astragalus injection affects the expression of cyt-c and CcO following oxygen-glucose deprivation and reintroduction in rat hippocampal neurons[J].Chinese Pharmacological Bulletin,2011,27(2):211-215. |
| |
| Authors: | JIAO Jun-xia GAO Wei-juan QIAN Tao ZHU Yan-jie DONG Ya-jie |
| |
| Institution: | JIAO Jun-xia,GAO Wei-juan,QIAN Tao,ZHU Yan-jie,DONG Ya-jie(Dept of Pathophysiology Chengde Medical College,Chengde Hebei 067000,China) |
| |
| Abstract: | Aim To investigate the effect of astragalus injection on the expression of cyt-c and CcO after hypoxia /hypoglycemia and reoxygenation in hippocampal neurons of rats.Methods The hippocampal neurons cultured for eight days were divided into 4 groups:normal control group,model group(hypoxia/hypoglycemia and reoxygenation group),original astragalus injection group,astragalus injection group.Model group,astragalus injection group and original astragalus injection group were treated with hypoglycemia and reoxyge... |
| |
| Keywords: | cyt-c CcO |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
