| 沙蟾毒精抑制肝癌HepG2细胞黏附、迁移和侵袭的作用 |
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| 引用本文： | 曹惠慧,张冬梅,刘俊珊,侯春英,栗原博,叶文才.沙蟾毒精抑制肝癌HepG2细胞黏附、迁移和侵袭的作用[J].中国药理学通报,2011,27(1):19-23. |
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| 作者姓名： | 曹惠慧 张冬梅 刘俊珊 侯春英 栗原博 叶文才 |
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| 作者单位： | 暨南大学药学院中药及天然药物研究所;中药药效物质基础及创新药物研究广东省高校重点实验室,广东,广州,510632 |
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| 基金项目： | 国家杰出青年科学基金资助项目,国家自然科学基金资助项目,广东省自然科学基金资助项目 |
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| 摘 要： | 目的研究沙蟾毒精(Arenobufagin)对人肝癌细胞HepG2黏附、迁移和侵袭的抑制作用。方法 MTT法检测沙蟾毒精对HepG2细胞活力和细胞黏附能力的影响,划痕实验观察沙蟾毒精对HepG2细胞运动能力的影响,Transwell小室模型研究沙蟾毒精对HepG2细胞的迁移和侵袭的抑制作用,Western blot检测基质金属蛋白酶MMP 2和MMP 9的表达水平变化。结果与空白组相比,沙蟾毒精可降低HepG2细胞的黏附能力,使Transwell小室膜上的肝癌细胞明显减少,并且呈剂量依赖性。Western blot结果显示沙蟾毒精可以明显抑制基质金属蛋白酶MMP 2和MMP 9的表达。结论沙蟾毒精能抑制人肝癌细胞HepG2的黏附、迁移和侵袭,其机制可能与抑制MMP 2和MMP 9的表达有关。
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| 关 键 词： | 沙蟾毒精 黏附 迁移 侵袭 基质金属蛋白酶 HepG2细胞 |
Inhibitory effect of Arenobufagin on the adhesion, invasion and migration of human hepatoma carcinoma cells |
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| CAO Hui-hui,ZHANG Dong-mei,LIU Jun-shan,HOU Chun-ying,KURIHARA Hiroshi,YE Wen-cai.Inhibitory effect of Arenobufagin on the adhesion, invasion and migration of human hepatoma carcinoma cells[J].Chinese Pharmacological Bulletin,2011,27(1):19-23. |
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| Authors: | CAO Hui-hui ZHANG Dong-mei LIU Jun-shan HOU Chun-ying KURIHARA Hiroshi YE Wen-cai |
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| Institution: | CAO Hui-hui1,2,ZHANG Dong-mei1,LIU Jun-shan1,HOU Chun-ying1,KURIHARA Hiroshi1,YE Wen-cai1,2(1.Institute of Traditional Chinese Medicine & Natural,2.Guangdong Provincial Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research,Jinan University,Guangzhou 510632,China) |
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| Abstract: | Aim To investigate inhibitory effects of Arenobufagin on the adhesion,invasion and migration of hepatoma carcinoma HepG2 cells.Methods The viability of HepG2 cells was measured by MTT assay.Cell adhesion assay,cell scratch assay and Transwell chamber assay were used to determine changes in adhesion,invasion and migration of HepG2 cells after Arenobufagin treatment.Western blot was used to detect the expression of matrix metalloproteinase 2 and 9(MMP 2 and MMP 9).Results Arenobufagin could obviously suppress... |
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| Keywords: | arenobufagin adhesion migration invasion MMPs HepG2 cells |
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