复肝灵对兔四氯化碳肝损伤后的影响

目的：观察中药制剂复肝灵颗粒对兔CCl4肝损伤的治疗作用。方法：采用10% CCl4油溶液5?ml/kg皮下注射建立兔实验性肝损伤模型，设生理盐水对照组、CCl4模型组、复肝灵（FGL）高、中、低剂量治疗组，对血清谷草转氨酶（AST）、谷丙转氨酶（ALT）、r 谷氨酰基转移酶（r GT）、总蛋白、白蛋白、球蛋白进行检测研究。结果：CCl4模型组体重明显降低，与生理盐水对照组相比有显著的统计学意义（P<0.01），FGL治疗组体重有所降低，但与生理盐水对照组相比无统计学意义（P>0.05）； CCl4模型组兔血清AST、ALT和r GT较生理盐水对照组和3个FGL治疗组显著增（P<0.01）； FGL?3个剂量组均能明显降低CCl4所引起的肝组织丙二醛(MDA)的升高（P<0.01）；在光学和电子显微镜下显示的组织学结构，FGL治疗组与对照组相比均有明显的修复。结论：复肝灵对兔CCl4肝损伤有良好的保护作用。

Experiment study on the effect of Fuganling on liver injury induced by CCl4 in rabbit

To study the effect of traditional Chinese medicine “Fuganling” （FGL）on liver injury induced by CCl4 in rabbits. Methods: Experimental liver injury models of rabbits were established by hyodermic injection of CCl4 oil solution(10%, 5?ml/kg), and then they were distributed to pumping brine group(control), CCl4 group, and high, median, low dose FGL group. The blood serum aspartate aminotransferase (AST), the glutamic pyruvic transaminase(ALT), the r glutamltransferase(r GT), the gross protein, the albumin, and the globin were tested 2 months later. Results: (1) The body weights decreased obviously in CCl4 indued injury group compared with brine group（P<0.01），　while they decreased unconspicuously in FGL therapy groups compared with brine groups（P>0.05）;　(2) Levels of serum AST, ALT and　r GT in CCl4 induced injury groups increased obviously compared with FGL therapy groups （P<0.01）; (3) CCl4 indued liver MDA enhance was decreased by FGL evidently （P<0.01）; (4) Microscope and electron microscopy revealed that the liver structure had been ameliorated in Fuganling treatment groups. Conclusion: Fuganling has obviously protection effect on liver injury.