| 高效液相色谱法测定蒙成药德都红花七味丸中没食子酸的含量 |
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| 引用本文： | 何春龙,王焕芸,莎日娜. 高效液相色谱法测定蒙成药德都红花七味丸中没食子酸的含量[J]. 中南药学, 2013, 11(2): 144-146 |
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| 作者姓名： | 何春龙 王焕芸 莎日娜 |
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| 作者单位： | 何春龙 (内蒙古医科大学药学院,呼和浩特,010110)；王焕芸 (内蒙古医科大学药学院,呼和浩特,010110)；莎日娜(内蒙古医科大学第二附属医院药剂科,呼和浩特,010030)； |
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| 基金项目： | 内蒙古自治区高等学校科学研究项目（编号：NJZY12144） |
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| 摘 要： | 目的研究和建立高效液相色谱法测定德都红花七味丸中没食子酸的含量方法。方法采用大连伊利特Hypersil ODS2柱（250 mm×4.6 mm,5μm）,以甲醇-0.1%磷酸溶液（4：96）为流动相,流速1.0 mL min^-1,检测波长为273 nm。结果没食子酸在2.048～20.48μg mL^-1与峰面积具有良好的线性关系,r=0.999 2,平均加样回收率为102.4%,RSD为1.6%。德都红花七味丸中没食子酸的含量≥0.111 6 mg/丸。结论本方法简便、准确、重复性好,可用于蒙成药德都红花七味丸中没食子酸的质量控制标准。
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| 关 键 词： | 德都红花七味丸 诃子 没食子酸 高效液相色谱法 |
HPLC determination of gallic acid in Dedu Honghua Qiwei pellets |
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| HE Chun-long,WANG Huan-yun,SHA Ri-na. HPLC determination of gallic acid in Dedu Honghua Qiwei pellets[J]. Central South Pharmacy, 2013, 11(2): 144-146 |
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| Authors: | HE Chun-long WANG Huan-yun SHA Ri-na |
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| Affiliation: | 1. Department of Pharmacy, Inner Mongolia Medical University, Hohhot 010110; 2. Department of Pharmacy, Second Affiliated Hospital, Inner Mongolia Medical University, Hohhot 010030) |
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| Abstract: | Objective To establish an HPLC method for the determination of gallic acid in Dedu Honghua Qiwei pellets. Methods Hypersil ODS2 （250 min×4.6 mm, 5 μm） column was used. The mobile phase was methanol-0.1% phosphoric acid （4 ： 96, v/v）, the flow rate was 1.0 mL .min^-1, and the detection wavelength was 273 nm. Results A good linearity of gallic acid was at 2.048 - 20.48 μg . mL^ - 1, r = 0.999 2）. The average recovery was 102.4% and RSD was 1.6%. The content of gallic acid in Deduhonghua Qiwei pellets was no less than 0.111 6 mg/pellets. Conclusion This method is simple, and accurate with good reproducibility, which can be used for the quality control of gallic acid in Dedu Honghua Qiwei pellets. |
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| Keywords: | Dedu Honghua Qiwei pellet Chebulae.fructus gallic acid HPLC |
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