星点设计-响应面法优化川芎生物碱提取工艺

目的优选川芎总生物碱的提取工艺。方法对川芎采用乙醇回流提取的方法,进行星点设计,以乙醇浓度、醇倍数、提取时间为自变量,以川芎中总生物碱含量为因变量,通过对自变量各水平的多元线性回归及二项式拟合,用响应面法优选出最佳提取工艺,同时进行预测分析。结果确定了最佳提取工艺为用8倍90%的乙醇提取2次,每次2 h,提取的川芎总生物碱含量为0.693 mg.g^-1,二项式拟合复相关系数R^2=0.957 3。结论星点设计-响应面法优选川芎中总生物碱的提取工艺,方法简单,精密度高,可预测性好。

Optimization of extraction technology for total alkaloids from Ligusticum chuanxiong by central composite design/response surface methodology

Objective To optimize the extraction process of total alkaloids from Ligusticum chuanxiong. Methods Independent variables were ethanol concentration, reflux time and solvent fold, and dependent variable was extraction rate of total alkaloids from Ligusticum chuanxiong. Levels of independent variables were regressed by multiple linearity and fitted by binomial. Response surface methodology was used to optimize the extraction process. We compared both observed and predicted values. Results Optimal extraction technology was： extracting twice by 8 times of 90% ethanol for 2 h each time. The optimum extraction process of total alkaloid content in Ligusticurn chuanxiong was under 0.693 mg· g^-1. Regression coefficients of binomial fitting complex model was as high as 0.957 3. Conclusion This extraction process of total alkaloids from Ligusticum chuanxiong by central composite design/response surface methodology is simple with high precision and good predictability.