氟比洛芬酯对大鼠机械通气所致肺损伤的影响

目的 探讨氟比洛芬酯对大鼠机械通气所致肺损伤的影响.方法 健康成年雄性SD大鼠40只,体重300～350 g,随机分为4组(n=10),常规潮气量通气组(TV组,潮气量8 ml/ks)、大潮气量通气组(HV组,潮气量40 ml/ks)、大潮气量通气+氟比洛芬酯5 ms/kg组(HV+F1组)和大潮气量通气+氟比洛芬酯10 mg/kg组(HV+F2组).HV+F1组和HV+F2组于机械通气前15 min时分别静脉注射氟比洛芬酯5、10 mg/ks.于机械通气4 h时处死大鼠,测定支气管肺泡灌洗液(BALF)肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、血栓素B2(TXB2)和总蛋白浓度,计数白细胞及计算肺组织湿/干重比(W/D),光镜下观察肺组织病理学结果.结果 与TV组相比,HV组BALF TNF-α、IL-6、TXB2、总蛋白浓度、白细胞计数和肺组织W/D升高(P<0.05),肺组织发生病理学损伤;与HV组相比,HV+F1组和HV+F2组BALF TNF-α、IL-6、TXB2、总蛋白浓度、白细胞计数和肺组织W/D降低(P<0.05),肺组织病理学损伤减轻;HV+F2组BALF TNF-α和TXB2浓度低于HV+F1组(P<0.05),其余指标差异均无统计学意义(P>0.05).结论 氟比洛芬酯可通过抑制炎性反应减轻大鼠机械通气所致肺损伤.

Effects of flurbiprofen on ventilator-induced lung injury in rats

Objective To investigate the effects of flurbiprofen on ventilator-induced lung injury in rats. Methods Forty healthy adult male SD rats weighing 300-350 g were randomly divided into 4 gorups ( n = 10 each) : group A traditional tidal volume (VT= 8 ml/kg) ; group B high tidal volume (HV, VT = 40 ml/kg) ; group C HV + flurbiprefen 5 ms/kg and group D HV + flurbiprofen 10 ms/kg. The animals were anesthetized with intraperitonel 20% urethane 8 ml/kg, tracheostomized and mechanically ventilated. In group C and D flurbiprofen was given iv at 15 rain before mechanical ventilation. The animals were mechanically ventilated for 4 h and then sacrificed. The lungs were removed for determination of TNF-α, IL-6, TXB2 total protein concentrations and WBC count in bronchoalveolar lavage fluid (BALF), W/D lung weight ratio and microscopic examination. Results The TNF-α, IL-6, TXB2, total protein concentrations, the number of WBCs and W/D ratio were significantly higher and the histologic damage was significantly more serious in group B than in group A. Flurbiprofen significantly attenuated the HV-induced changes in group C and D. The TNF-α, TXB2 concentrations in BALF were significantly lower in group D than in group C. Conclusion Fhrbiprofen can attenuate ventilator-induced lung injury in rats.