Caspase抑制剂对特应性皮炎患者外周血CD4+T细胞亚群分泌细胞因子的影响

目的：明确广谱半胱氨酸天冬氨酸蛋白酶(caspase)抑制剂对特应性皮炎(AD)患者外周血CD4+T细胞亚群分泌细胞因子的影响。方法：广谱caspase抑制剂Z-VAD-FMK与30例AD患者外周血单一核细胞(PBMCs)体外共培养后,PBMCs分为3组，分别加入Z-VAD-FMK溶液、地塞米松溶液和PBS溶液。流式细胞仪检测CD4+T细胞亚群；ELISA方法检测上清液中IFN-γ、IL-4、IL-17的浓度。结果：IFN-γ、IL-4、IL-17水平在PBS溶液组高于Z-VAD-FMK组，差异均有统计学意义(均P<0.01)，在Z-VAD-FMK组与地塞米松组间比较，均无统计学意义(均P>0.05)。结论：广谱caspase抑制剂Z-VAD-FMK可抑制AD患者PBMC中CD4+T细胞Th1、Th2、Th17分泌相关细胞因子。

Effect of Z-VAD-FMK,a broad caspase inhibitor,on the expression of cytokines released by CD4+T cell subset in the patients with atopic dermatitis

Objective: To determine the effect of Z-VAD-FMK, a broad caspase inhibitor, on the expression of cytokines released by CD4+T cells subset in the patients with atopic dermatitis (AD). Methods: PBMCs from 30 AD patients were co-cultured with Z-VAD-FMK solution in vitro, then the cells were divided into PBS group, Z-VAD-FMK group and dexamethasone group. The levels of CD4+T cells were detected by flow cytometry. The level of IFN-γ, IL-4 and IL-17 were detected by ELISA. Results: The levels of IFN-γ, IL-4 and IL-17 in the PBS group were higher than that in the Z-VAD-FMK group (Ps<0.01) and there was no significant differences of these between the Z-VAD-FMK group and dexamethasone group (Ps>0.05). Conclusion: Z-VAD-FMK can inhibit the expression of cytokines released by CD4+T cells subset of Th1, Th2 and Th17 in patients with AD.