封闭群比格犬遗传标志微卫星引物的筛选

目的筛选出有效的微卫星引物，利用微卫星遗传标志对封闭群内比格犬的个体遗传情况进行分析。方法从文献中筛选杂合度在0．4～0．8的微卫星引物24对，对本场英系比格犬封闭群中随机筛选出的12只比格犬的混合基因组进行了扩增。结果24对引物中，有7对微卫星引物的扩增结果具有较好的多态性和重复性。利用这7对引物，对8只玛斯公司美系比格犬的混合基因组进行了扩增，结果与本场英系比格犬的扩增结果具有明显的差异。又利用这7对引物对随机筛选出的6只比格犬的个体遗传情况进行了分析，其中3只比格犬表现出明显的差异。结论作者筛选的7对微卫星引物可以对本场比格犬种群的个体遗传情况进行初步分析。

Screening of Microsatellite Primers Used for Genetic Mark of Closed Colony in Beagle Dogs

Objective To select the effective microsatellite primers and analyze the genetic circumstance of individual Beagle dogs. Methods Twenty-four pair of primers with the heterozygotic degree between 0.4-0.8 were selected from the literature. The primers were authenticated used the mix-genome of 10 Beagle dogs. Results Among the 24 pair primers, 7 pair of primers were polymorphism and reproducibility. The 7 pair of primers used the mix-genome of 10 Beagle dogs of MaSi corporation were also tested, The results showed obviously different from Beagle dogs of our plant. The genetic circumstance of 6 individual Beagle dogs were also analyzed used the same 7 pair primers, four of them showed obviously different. Conclusion The 7 pair primers were suitable for analyzed the genetic circumstance of individual Beagle dogs primarily.