| 总量统计矩结合聚类分析与主成分分析评价虎杖饮片一致性与差异性 |
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| 引用本文： | 王元清,韩彬,向荣,孙琴,张陈,乔志斌,周钰珂,严建业,夏新华.总量统计矩结合聚类分析与主成分分析评价虎杖饮片一致性与差异性[J].中草药,2015,46(19):2863-2869. |
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| 作者姓名： | 王元清 韩彬 向荣 孙琴 张陈 乔志斌 周钰珂 严建业 夏新华 |
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| 作者单位： | 中南林业科技大学生命科学与技术学院 生物技术与工程实验室, 湖南 长沙 410004;广东药学院中药学院, 广东 广州 510006;中南林业科技大学生命科学与技术学院 生物技术与工程实验室, 湖南 长沙 410004;中南林业科技大学生命科学与技术学院 生物技术与工程实验室, 湖南 长沙 410004;暨南大学药学院, 广东 广州 510632;中南林业科技大学生命科学与技术学院 生物技术与工程实验室, 湖南 长沙 410004;中南林业科技大学生命科学与技术学院 生物技术与工程实验室, 湖南 长沙 410004;湖南中医药大学药学院 中药现代化重点实验室, 湖南 长沙 410208;湖南中医药大学药学院 中药现代化重点实验室, 湖南 长沙 410208 |
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| 基金项目： | 国家自然科学基金面上项目(81173194);湖南省中药活性物质筛选工程技术研究中心项目资助(湘科计字[2013]83号);湖南省高校"中药新药创制与资源综合持续利用"科技创新团队资助(湘教通[2010]212号);湖南省"中药学"重点学科资助(湘教通[2011]76号);国家级大学生创新创业训练计划项目(201410538015);湖南省大学生研究性学习与创新性实验计划项目(2014194);中南林业科技大学大学生研究性学习与创新性实验计划项目(201457) |
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| 摘 要： | 目的评价不同批次虎杖饮片一致性与差异性,为其质量控制提供方法参考。方法采用HPLC法建立11批虎杖饮片的化学指纹图谱;采用总量统计矩法计算总量零阶矩(AUCT)、总量响应率(AUCPWT)、总量一阶矩(MCRTT)、总量二阶矩(VCRTT)等参数;采用总量统计矩法、夹角余弦法和相关系数法进行相似度评价;采用聚类分析法与主成分分析(PCA)法对虎杖样品进行分类分析。结果对11批虎杖饮片进行测定,共标定了13个共有峰,指认了虎杖苷、白藜芦醇、大黄素苷、大黄素的色谱峰;总量统计矩参数AUCT、AUCPWT、MCRTT、VCRTT的平均值分别为1.52×106 AU·min、2.31×103 AU·min·mL/mg、22.0 min、110.0 min2;各批次样品的相似度在0.85以上;聚类分析法与PCA法均将样品分为4类。结论化学指纹图谱结合总量统计矩分析、聚类分析与PCA可评价虎杖饮片一致性与差异性,是适合中药特点的质量控制方法。
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| 关 键 词： | 虎杖 一致性 差异性 指纹图谱 总量统计矩 聚类分析 主成分分析 虎杖苷 白藜芦醇 大黄素苷 大黄素 |
| 收稿时间： | 2015/5/16 0:00:00 |
Evaluation of consistency and difference of Polygoni Cuspidati Rhizoma et Radix pieces using total statistical moment method combined with cluster analysis and principal component analysis |
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| WANG Yuan-qing,HAN Bin,XIANG Rong,SUN Qin,ZHANG Chen,QIAO Zhi-bin,ZHOU Yu-ke,YAN Jian-ye and XIA Xin-hua.Evaluation of consistency and difference of Polygoni Cuspidati Rhizoma et Radix pieces using total statistical moment method combined with cluster analysis and principal component analysis[J].Chinese Traditional and Herbal Drugs,2015,46(19):2863-2869. |
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| Authors: | WANG Yuan-qing HAN Bin XIANG Rong SUN Qin ZHANG Chen QIAO Zhi-bin ZHOU Yu-ke YAN Jian-ye and XIA Xin-hua |
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| Institution: | Laboratory of Biotechnology and Engineering, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China;School of Chinese Materia Medicia, Guangdong Pharmaceutical College, Guangzhou 510006, China;Laboratory of Biotechnology and Engineering, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China;Laboratory of Biotechnology and Engineering, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China;School of Pharmacy, Jinan University, Guangzhou 510632, China;Laboratory of Biotechnology and Engineering, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China;Laboratory of Biotechnology and Engineering, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China;Key Laboratory of Modernization of Chinese Materia Medicia, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;Key Laboratory of Modernization of Chinese Materia Medicia, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China |
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| Abstract: | Objective To establish a method to evaluate the consistency and difference of Polygoni Cuspidati Rhizoma et Radix from different batches. Methods Chemical fingerprints of 11 batches of Polygoni Cuspidati Rhizoma et Radix were established by HPLC; The follow parameters, such as under curve of total quantum (AUCT), area under curve of total quantum perweight (AUCPWT), mean chromatographic retention time of total quantum (MCRTT), and variance of chromatographic retention time of total quantum (VCRTT), were also calculated using total statistical moment method. The similarities were obtained by total statistical moment method, cosine method, and correlation coefficient method. Cluster analysis (CA) and principal component analysis (PCA) were adopted to classify the samples. Results Chemical fingerprints were established with 13 common peaks and polydatin, resveratrol, emodin glycosides, and emodin were identified. AUCT, AUCPWT, MCRTT, and VCRTTwere calculated as 1.52×106 AU·min, 2.31×103 AU·min·mL/mg, 22.0 min, and 110.0 min2, respectively. The similarities of all batches of samples were over 0.85. The samples were divided into four categories by CA or PCA. Conclusion Chemical fingerprint combined with total statistical moment method, CA, and PCA can evaluate the consistency and difference of Polygoni Cuspidati Rhizoma et Radix, which is suitable for the quality control of Chinese materia medica. |
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| Keywords: | Polygonum cuspidatum Sieb et Zucc consistency difference fingerprint total statistical moment method cluster analysis principal component analysis polydatin resveratrol emodin glycosides emodin |
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