云南个旧、宣威地区肺癌端粒酶hTR检测分析

目的探讨云南个旧、宣威地区肺癌端粒酶hTR的表达及与其发病机理的关系.方法用原位杂交方法检测端粒酶hTR在上述两地肺癌中的表达情况.其中个旧矿工肺癌24例,宣威农民肺癌36例,其他地区肺癌21例.结果81例肺癌中hTR阳性率为65.4%(53/81),其中个旧矿工肺癌66.7%(16/24),宣威农民肺癌58.3%(21/36),其他地区肺癌76.2%(16/21).癌旁正常支气管上皮和肺泡上皮中未检出hTR,而在部分病例(12.3%,10/81)的癌旁增生肺泡Ⅱ型上皮中检测出hTR.3个地区hTR的表达阳性率未见显著性差异(P＞0.05).hTR的表达与性别、年龄及组织分化程度无关(P＞0.05),与组织学类型及淋巴结转移有关(P＜0.05).结论端粒酶hTR的表达在3个地区肺癌中具有普遍性,可作为肺癌标志物.

Human telomerase RNA (hTR) expression in lung cancer in the Gejiu and Xuanwei regions of Yunnan

OBJECTIVE: To investigate the correlation between the expression of hTR in and the pathogenesis of lung cancer in Gejiu and Xuanwei regions of Yunnan province. METHODS: By use of in situ hybridization, the expression of hTR gene was observed in 81 cases of lung cancer tissues including 24 cases of Gejiu Tin miners (GTM), 36 cases of Xuanwei farmers (XF) and 21 cases of other regions. RESULTS: hTR was detected in 65.4% (53/81) of the lung cancer cases. The positive rate of hTR in GTM, XF and other regions was 66.7% (16/24), 58.3% (21/36) and 76.2% (16/21) respectively. Positive hTR expression was not found in normal bronchial and alveolar epithelia, but was found in highly proliferating type II alveolar epithelial cells adjacent to the carcinoma (12.3%, 10/81). No significant difference was observed with regard to the positive rate of hTR expression among three different regions. There was no statistically significant correlation between the expression of hTR, the patients' sex, age and the differentiation degree of the tumor, but the expression of hTR was correlated with histological type and lymph node metastasis (P < 0.05). CONCLUSION: The expression of hTR is a prevailing event in lung cancer and therefore can be used as an indicator of lung malignancy.