P21、P185、P53蛋白表达及P53基因突变与膀胱癌移行细胞生物学行为关系的研究

目的：探讨癌基因及抑癌基因改变与膀胱癌生物学行为产关系。方法：采用免疫组织化学方法及分子生物学技术，检测了８２例膀胱移行细胞癌Ｐ２１、Ｐ１８５、Ｐ５３蛋白表达及３８例Ｐ５３基因突变。结果：Ｐ２１、Ｐ１８５、Ｐ５３蛋白表达阳性率分别为６２．２％（５１例）、５８．５％（４８例）、３１．７％（２６例）。１２例（３１．６％）有Ｐ５３抑癌基因突变，其中全部有第２８０位密码子Ｇ－Ｃ突变；３例合并有２４８密码子

Relation of expression of P21, P185 and P53 proteins and point mutation of P53 gene to prognosis of cystic transitional cell carcinoma

Objective: To investigate the relationship between changes of oncogenes and antioncogenes and biological behaviors of cystic carcinoma. Methods: The expression of P21, P185 and P53 and mutation in P53 gene were studied in 82 cases of cystic transitional cell carcinoma by immunohistochemical and molecular biological methods. Results: The positive rates of P21, P53 and P185 were 62.2%, 31.7% and 58.5% respectively. The rates of P21 and P53 were increased with the progression of the pathological grade and the clinical stage (P<0.01) while that of the P185 decreased with them (P<0.05). The point mutation rate in P53 gene was 31.6%. All the P53 point mutation positive cases had G C transition at codon 280. Point mutation at codons 248 and 249 were detected in 3 and 1 respectively among the 12 P53 point mutation positive cases. Conclusion: The positive expression of p21 and p53 protein was positively correlated to the pathological grading and clinical staging of transitional cell carcinoma (P<0.01) while that of p185 was negatively correlated to the grading and staging of the carcinoma (P<0.05). The point mutation of P53 antioncogene is closely related to the high grade and/or high stage subsets of bladder cancer. The point mutation positive cases have a higher recurrent rate and death rate.