Accelerated development of human monocyte-macrophages cultured on Plastek-C tissue culture dishes

Summary Many investigators have found that the surface on which cells are cultured can be an important factor in their adherence and subsequent growth. To enhance the development of monocyte-macrophage cultures, we have grown these cells on polystyrene tissue cultureware that has been treated with gas plasma to induce a positively charged culture surface (Plastek-C cultureware from Tekmat). Human monocyte-macrophages grown on Plastek-C tissue cultureware demonstrated a greater cell protein content during culture development as compared to monocyte-macrophages grown on standard polystyrene tissue cultureware. Part of the reason for enhanced development of human monocyte-macrophage cultures on Plastek-C cultureware seemed to be due to a higher density of cells maintained during the initial stages of culture development.