| Expression of TGF-β2 mRNA and PCNA, FN Protein in Lens Epithelial Cells in Age-related Nuclear and Cortex Cataract |
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| 引用本文： | 叶琳,李琴,蔡小军,邓平.Expression of TGF-β2 mRNA and PCNA, FN Protein in Lens Epithelial Cells in Age-related Nuclear and Cortex Cataract[J].华中科技大学学报(医学英德文版),2005,25(5):578-581. |
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| 作者姓名： | 叶琳 李琴 蔡小军 邓平 |
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| 作者单位： | Department of Ophthalmology, Zhongnan Hospital. Wuhan University,Wuhan 430071, China |
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| 基金项目： | This project was supported by a grant from Natural Sciences Foundation of Hubei Province （No. 2000J059）. |
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| 摘 要： | Summary： By using RT PCR and immunohistochemistry, the expressions of transforming growth factor 132 （TGF-β2） mRNA, proliferating cell nuclear antigen （PCNA） and fibronection （FN） protein in lens epithelial cells （LECs） of age-related nuclear and cortex cataract were detected and compared. The results of RT-PCR revealed that the expression of TGF-β2 mRNA was higher in cortex cataract than in nuclear cataract. Immunohistochemistry demonstrated that the expression of PCNA protein was lower and the expression of FN protein was higher in cortex cataract than in nuclear cataract. It was suggested that TGF-β2 , PCNA and FN might take important parts in the process of age-related cataract. Cortex cataract was related to the transdifferentiation of LECs, and nuclear cataract to the proliferation of LECs.
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| 关 键 词： | TGF-β2 mRNA PCNA 基因表达 FN蛋白质 上皮细胞 白内障 年龄 |
| 收稿时间： | 2004-11-18 |
Expression of TGF-β2 mRNA and PCNA, FN protein in lens epithelial cells in age-related nuclear and cortex cataract |
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| Ye Lin,Li Qin,Cai Xiaojun,Deng Ping.Expression of TGF-β2 mRNA and PCNA, FN protein in lens epithelial cells in age-related nuclear and cortex cataract[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(5):578-581. |
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| Authors: | Ye Lin Li Qin Cai Xiaojun Deng Ping |
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| Institution: | (1) Department of Ophthalmology, Zhongnan Hospital, Wuhan University, 430071 Wuhan, China |
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| Abstract: | Summary By using RT-PCR and immunohistochemistry, the expressions of transforming growth factorβ
2(TGF-β2) mRNA, proliferating cell nuclear antigen (PCNA) and fibronection (FN) protein in lens epithelial cells (LECs) of age-related
nuclear and cortex cataract were detected and compared. The results of RT-PCR revealed that the expression of TGF-β2 mRNA was higher in cortex cataract than in nuclear cataract. Immunohistochemistry demonstrated that the expression of PCNA
protein was lower and the expression of FN protein was higher in cortex cataract than in nuclear cataract. It was suggested
that TGF-β2, PCNA and FN might take important parts in the process of age-related cataract. Cortex cataract was related to the transdifferentiation
of LECs, and nuclear cataract to the proliferation of LECs.
YE Lin, female, born in 1973, M. D., Ph. D.
This project was supported by a grant from Natural Sciences Foundation of Hubei Province (No. 2000J059). |
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| Keywords: | TGF-β 2 PCNA FN LECs cataract cortex nuclear |
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