| 鼻咽癌体外传代细胞株SUNE中EBV-LMP_1全基因的扩增 |
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| 引用本文： | 陈宜芳,郭辉玉,汪慧民,李满枝.鼻咽癌体外传代细胞株SUNE中EBV-LMP_1全基因的扩增[J].中山大学学报(医学科学版),1997(Z1). |
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| 作者姓名： | 陈宜芳 郭辉玉 汪慧民 李满枝 |
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| 作者单位： | 中山医科大学微生物教研室 |
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| 摘 要： | 用长片段高保真度ＰＣＲ扩增系统（ｅｘｐａｎｄＴＭｈｉｇｈｆｉｄｅｌｉｔｙＰＣＲｓｙｓｔｅｍ）从鼻咽癌（ＮＰＣ）体外传代细胞株ＳＵＮＥ中扩增出ＥＢ病毒（ＥＢＶ）潜伏膜蛋白（ＬＭＰ１）全基因，经凝胶电泳分析和ＤＮＡ斑点杂交进行鉴定。扩增片段长度为３．４３ｋｂ，相当于Ｂ９５－８细胞ＥＢＶ基因序列位置为１７００３３～１６６６０８ｎｔ，包括ＬＭＰ１基因的上游启动子／增强子序列、ＬＭＰ１编码序列的３个外显子、两个内含子及下游ｐｏｌｙＡ信号序列。ＬＭＰ１全基因的扩增对于深入研究ＬＭＰ１基因的结构与功能以及ＬＭＰ１在ＮＰＣ发生发展中的作用机制具有十分重要的价值。
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| 关 键 词： | 鼻咽肿瘤／遗传学 疱疹病毒4型.人／遗传学 基因.病毒／遗传学 病毒基质蛋白质类／生物合成 |
AMPLIFICATION OF LATENT MEMBRANE PROTEIN GENE OF EPSTEIN BARR VIRUS FROM NASOPHARYNGEAL CARCINOMA CELL LINE SUNE |
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| Chen Yifang,Guo Huiyu,Wang Huimin,Li Manzhi.AMPLIFICATION OF LATENT MEMBRANE PROTEIN GENE OF EPSTEIN BARR VIRUS FROM NASOPHARYNGEAL CARCINOMA CELL LINE SUNE[J].Journal of Sun Yatsen University(Medical Sciences),1997(Z1). |
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| Authors: | Chen Yifang Guo Huiyu Wang Huimin Li Manzhi |
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| Institution: | Chen Yifang 1 Guo Huiyu 1 Wang Huimin 2 Li Manzhi 2 |
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| Abstract: | Latent membrane protein(LMP 1)gene of Epstein Barr virus(EBV)was amplified from propagated nasopharyngeal carcinoma(NPC)cell line SUNE by Expand TM High Fidelity PCR System.PCR products were identified by agarose gel electrophoresis analysis and DNA dot blot hybridization.The length of the amplified fragment was 3.43 kb,corresponding to EBV gene coordinates 170 033 nt to 166 608 nt of B 95 8 cell,containing LMP 1 gene promoter/enhancer region,the coding sequence(3 exons and 2 introns)and poly A signal.We believed that the amplification of entire LMP 1 gene was valuable to study further the structure and function of LMP 1 gene and its possible mechanism contributed to the occurrence and development of NPC. |
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| Keywords: | nasopharyngeal neoplasms/genetics herpesvirus 4 human/genetics genes virus/genetics viral matrix proteins/biosynthesis |
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