原发性肝细胞癌p53蛋白和多药耐药相关蛋白的表达及意义

目的探讨原发性肝细胞癌中P-gp、p53蛋白的表达及相互关系。方法应用免疫组织化学S-P法检测37例原发性肝细胞癌及19例非肝癌肝组织中p53蛋白与P-gp的表达。结果37例原发性肝细胞癌P-gp及p53蛋白的表达率为81.08%和64.86%,19例非肝癌肝组织P-gp及p53蛋白的表达率为42.10%和36.84%,相对应的两者有显著性差异(P<0.05);在原发性肝细胞癌中,P-gp的表达与远处转移相关,p53蛋白的表达与原发性肝细胞癌远处转移、肝内转移以及肿瘤的Edmondson分级相关(P<0.05),而且P-gp表达与p53蛋白的表达间具有相关性(P<0.05)。结论原发性肝细胞癌的P-gp及p53蛋白的表达,对于临床上认识原发性肝细胞癌的生物学特性,选择合理的化疗用药及估计预后具有重要意义。

Expression and significance of p53 protein and P - glycoprotein in hepatocelluar carcinoma

Objective To evaluate the expression of p-gpand p53 protin in hepatocellular carcinoma (HCC) and to explore the relationship between the expression of P-gp and p53 protein . Methods Expression of P-gp and p53 protein in 37 histologically verified HCC and 19 non-HCC specimens were examined by immunohistochemical methods. Results The positive rates of P-gp and p53 protein expression were 81.08 and 64.86% respectively in HCC tissues- it`s higher than P-gp and p53 protein expression in non-HCC liver tisssues(P<0.05). The expression of P-gp was related to AFP, HBV infection and distant metastasis. The expression of p53 protein was related to histological grade, local metastasis and distant metastasis(P<0.05). There was positive correlation between p53 protein and P-gp expression in HCC. Conclusion The expression of P-gp and p53 protein in HCC played an important role in chemotherapy strategy , metastasis of HCC and predictor of biological behavior and prognosis.