IHC和FISH检测乳腺癌HER2基因表达的比较研究

目的探讨荧光原位杂交（FISH）与免疫组织化学（IHC）检测HER2基因状态的结果的一致性及HER2表达与其淋巴结转移的关系及意义。方法采用IHC技术检测68例乳腺浸润性导管癌HER2基因的表达，同时进行FISH检测。结果HER2表达（＋）的28例标本中，其中27例经FISH检测无扩增，1例有扩增；HER2表达（＋＋）的12例标本中，7例经FISH检测无扩增，2例有扩增，另3例为可疑；HER2表达（＋＋＋）的28例标本中，经FISH检测均扩增；FISH检测有扩增的30例标本中，26例发生了淋巴结转移；可疑的3例中有2例有淋巴结转移；无扩增的34例标本中只有6例发生了淋巴结转移。结论当HER2表达为（＋）或（＋＋＋）时。IHC和FISH检测的一致性较高，而当HER2表达为（＋＋）时，必须进行FISH检测；HER2的扩增与淋巴结的转移关系密切，呈正相关（P〈0．05）。

A Comparative Study on HER-2 Expression by Immunohistochemistry and Fluorescence in Situ Hybridization in Breast Cancer Tissue

Objective To explore the relationship between HER-2 gene expression and lymph node metastasis by Fluorescence in Situ Hybridization （ FISH）, and to compare these results with those obtained by immunohistocbemistry （IHC）. Methods IHC and HSH were employed to determine the expression of HER-2. Results Among 28 samples with HER-2 expression score （ ＋ ） by IHC, 27 were negative for HER-2 expression by FISH and only one was positive. Among 12 samples with score （ ＋ ＋ ） by IHC, 7 were negative and 2 were positive by HSH, remaining 3 uncertain. All of the 28 samples with HER-2 expression score （ ＋ ＋ ＋ ） by IHC were also positive by FISH. In addition, 26 of the 30 cases with positive HER-2 expression by FISH were identified with lymphatic metastasis; 2 of the 3 uncertain cases were identified with lymph node metastasis; however, only 6 of the 34 cases with negative HER-2 expression by FISH were identified with lymph node metastasis. Conc/uslon A high consistent results are showed by IHC and IHC when HER-2 expression score is （ ＋ ） or （ ＋ ＋ ＋ ）. However, when the HER-2 expression score is （ ＋ ＋ ）, the results of these two methods are different, so HSH must be employed. Additionally, there is a positive correlation between HER-2 expression and lymph node metastasis （p 〈0. 05）.