微卫星技术在NIH小鼠群体遗传结构分析中的应用

目的应用微卫星DNA技术对A单位NIH小鼠和B单位NIH小鼠的遗传结构进行对比分析。方法利用30个微卫星位点对2个NIH小鼠种群进行PCR扩增和STR扫描,借助统计软件Popgene 1.32进行群体遗传结构分析。结果 A单位NIH群体获得74个等位基因型,平均杂合度为0.3108,多态性信息含量为0.2637;B单位NIH群体获得76个等位基因型,平均杂合度为0.3257,多态性信息含量为0.2777。群体间比较显示,群体间分化系数为0.3932,遗传一致性指数0.3971,遗传距离为0.9235,群体差异显著。结论两个NIH小鼠群体间存在严重的遗传分化,形成了两个不同的封闭群群体。

Application of microsatellite technology in the genetic structure analysis of NIH mice

Objective To compare and analyze the genetic structure of NIH mice bred in Unites A and B, using microsatellite technology. Methods Thirty SPF 8-week old outbred NIH mice (half male and half female) of each population were randomly chosen from the Units A and B, respectively. PCR amplification and STR scan were performed to determine the genetic characteristics of two outbred populations using microsatellite loci, and the population genetic structure was analyzed with statistical software Popgene 1.32. Results In the NIH mouse population form the Unit A, 74 alleles were obtained, with an average heterozygosity of 0.3108 and polymorphism information content of 0.2637. In the NIH mouse population from the Unit B, 76 alleles were obtained, with an average heterozygosity of 0.3257 and polymorphism information content of 0.2777. The inter-population comparison showed that genetic differentiation coefficient Fst was 0.3932, the genetic identity was 0.3971, and the genetic distance was 0.9235. The population difference was significant. Conclusions There is serious genetic differentiation between the two NIH mice populations, resulting in the formation of two different closed populations.