乙型肝炎病毒X基因转染人肝细胞致裸鼠的成瘤实验

目的 拟在裸鼠体内证实HBV X基因能否转化人肝细胞形成移植瘤.方法 利用高表达HBx基因的pCMVX/QSG7701细胞株作为实验组,以表达空白质粒的pRcCMV2/QSG7701细胞株和QSG7701细胞株为对照组接种于裸鼠皮下,观察裸鼠能否成瘤.HE染色、显微镜检查研究移植瘤的组织形态学特征.成瘤率的比较采用Fisher's精确检验.结果 接种pCMVX/QSG7701细胞株的所有6只裸鼠在第2周开始均出现移植瘤生长,周围器官和组织未发现转移灶.对照组至接种后第35天无移植瘤生长(X2-16.505,P＜0.01).HE染色证实移植瘤为肝细胞痛组织.结论 HBV X基因表达可以直接导致肝细胞癌变.

Study on tumor formation of hepatocyte transformed by hepatitis B virus X gene in nude mice

Objective To investigate whether hepatitis B virus X gene alone is sufficient to transform the non-transformed immortalized human liver cell line QSG7701 and induce hepatocellular carcinoma in vivo.Methods pCMVX/QSG7701 cells were transplanted into subcutaneous tissue of nude mice.pRcCMV2/QSG7701 and QSG7701 cells were used as contr01.Tumor formation was checked within 5 weeks after transplantation.The activity and food intake of the nude mice were recorded.The texture,volume and metastasis of transplantation tumor were observed grossly,and the HE stained transplantation tumor tissues were observed under optical microscope.Results The transplantation tumor occurred in all of the six nude mice inoculated with pCMVX/QSG7701 cells at the second week after inoculation.No metastatic tumor was found in other organs.Transplant tumor was not formed in all of the negative control groups.The activity,eating and drinking of the nude mice transplanted with pCMVX/QSG7701 cells were normal,while their weights were increased gradually in the first 3 weeks.Since the 4th week after transplantation with pCMVX/QSG7701 cells,the activity of the mice was decreased and their body weight was no longer increased.It is interesting that the mental state and eating ofthose nude mice inoculated with pRcCMV2/QSG7701and QSG7701 cells were normal,and the weight was increasing all the time after inoculation.HE staining analysis confirmed that the transplanted tumor was hepatocellular carcinoma.Conclusion HBx alone iS sufficient to transform the non-tranSformed immortalized human liver cellline QSG7701 and induce hepatocellular carcinoma in vivo.