溶栓颗粒对慢性脑供血不足大鼠脑组织细胞凋亡及Bcl-2 Fas-L蛋白表达的影响

目的:探讨溶栓颗粒对慢性脑供血不足大鼠的脑保护作用。方法:结扎双侧颈总动脉造成大鼠慢性脑供血不足模型,用TUNEL染色和免疫组化法分别检脑组织凋亡细胞及Bcl-2、Fas-L蛋白表达情况。结果:慢性脑供血不足可造成大鼠脑组织凋亡细胞增多,并有Bcl-2、Fas-L蛋白的高表达。与模型组比较,中药组脑细胞凋亡及Fas-L蛋白的表达较少Bcl-2蛋白表达增多。结论:溶栓颗粒可以保护慢性脑供血不足大鼠受损伤脑组织,并可能通过减少Fas-L蛋白,增加Bcl-2表达,从而减少脑组织细胞凋亡途径实现。

Effect of Rongshuan Grain on Injury Caused by CCCI of the Rats' Apoptosis and Protein Bcl-2 and Fas-L

Objective:To study the protective effect of Rongshuan Grain on injury caused by chronic cerebral circulation insufficiency(CCCI) of the rats.Methods:The CCCI rat model was established by ligating both of the cervical artery.At the same time,we observed the expression of rats' brain tissue' apoptosis and protein Bcl-2 and Fas-L by TUNEL dyeing and immunohistochemic.Results:CCCI increased the expression of rats' brain tissue' apoptosis and protein Bcl-2 and Fas-L.The apoptosis and the expression of protein Fas-L in Rongshuan Grain group was decreased.The expression of protein Bcl-2 was increased.Conclusion:Rongshuan Grain can protect the injury of the brain tissue of CCCI rats,and this function be likely to achieve from reduce apoptosis and the expression of protein Fas-L and increase the expression of protein Bcl-2.