分离人卵泡液中颗粒细胞两种方法的比较

目的：探讨简便可行分离卵巢颗粒细胞的方法以供化疗保护卵巢功能的体外实验研究。方法：分别采用密度梯度离心法和低渗透性溶解法分离及原代培养16例卵泡液中的颗粒细胞。结果：低渗透性溶解法与所密度梯度离心法分离及原代培养的细胞其细胞活率和雌激素的分泌量大体相当，差异无统计学意义（P>0.05）；但低渗透性溶解法耗时耗钱明显比密度梯度离心法低。差异有统计学意义(P<0.05)。结论：低渗透性溶解法并不降低颗粒细胞的活率及其激素的分泌，反而较密度梯度离心法省时省钱，简便高效。

Comparing two method for isolating and culturing ovarian granulosa cells

Purpose: To validate an expedited method for the removal of erythrocytes when preparing IVF granulosa-luteal cells for culture.Methods: Granulosa cells were enriched for culturing from follicular aspirates by density gradient centrifugation and by hypo-osmotic lysis treatments in 6 cases.Results: Cells prepared by either method showed the same cell viability and produced estrogen in similar quantities, there were no significant difference(P>0.05). But the time-consuming and money-consuming for density gradient centrifugation are higer than the hypo-osmotic lysis technique (P<0.01). Conclusions: Using hypo-osmotic lysis technique does not impair granulosa cell viability or steroidogenesis. It avoids multiple density gradient centrifugations and washings, and yields IVF granulosa cells ready for culture efficiently.