抗SPIM自身抗体的ELISA测定与临床应用

用ELISA 法定量检测生育、不育男性血清、精浆抗SPIM 自身抗体(SPIM-Ab)。结果表明:不育组和妻子流产组患者血清、精浆SPIM-Ab 含量均显著高于生育组(P<0.01),两组血清、精浆的SPIM-Ab 阳性率与生育组之间有显著性差异(P<0.01);精浆SPIM-Ab 含量高于5.0U/ml 时,精于密度、精子活率及精浆SPIM 活性均明显降低;SPIM-Ab 与血清、精浆抗精子抗体(AsAb)无明显关系。提示:SPIM-Ab 水平增加可能抑制SPIM 活性、与SPIM一起参与局部免疫反应,并减少精子密度和活率。

Detection of Anti-SPIM Autoantibodies by ELISA and Clinical Application

The serum and seminal plasma Anti-SPIM autoantibodies(SIPM-Ab)levels were determined by ELISA in fertility and infertility men.The results showed that the content of serum and seminal plasma SPIM-Ab in the infertile men and the men with coupled aborted wifegoups were all significantly higher than in the fertile group(P<0.01),there was a statisticaldifference between the positive rates of SPIM-Ab in serum and seminal plasma of patient groupand fertile group(P<0. 01).The sperm desity,viability and SPIM activity were significantlydecreased when the level of anti-SPIM antibodies in seminal plasma was 5.0U/ml or higher.SPIM-Ab showed no relationship with antisperm antibodies in serum and seminal plasma.Thisstudy indicated that the elevation of SPIM-Ab level might inhibit the SPIM activity and might beinvolved in the local immune reaction with SPIM and decrease the sperm density and viability.