Evaluation of the antinociceptive and anti-inflammatory effects of essential oil of Nepeta pogonosperma Jamzad et Assadi in rats |
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Authors: | Taskina Ali Mohammad Javan Ali Sonboli Saeed Semnanian |
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Affiliation: | 1.Department of Physiology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh;2.Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;3.Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran |
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Abstract: | Background and the purpose of studyConcerning the different effects of essential oils from Nepeta genus on the central nervous system including pain killing effect, this study was designed to evaluate the antinociceptive and anti-inflammatory effects of essential oil of Nepeta pogonosperma Jamzad et Assadi (NP), a recently identified species.MethodsAir-dried aerial parts of NP were hydrodistillated and GC-MS analysis of obtained essential oil was conducted. Total 24 male Wister rats weighing 225 ± 25 gm were studied. Essential oil of NP was administered intraperitoneally at the doses of 50 mg/kg, 100 mg/kg and 200 mg/kg for the experimental groups. Control rats received equal volume (2 ml/kg) of normal saline. Antinociception was assessed by tail flick test (after 30 minutes) and formalin test (for further 60 minutes). Then the animal was sacrificed and the paw edema was measured using a water plethysmometer.Results4aα,7α,7aβ-nepetalactone and 1,8-cineole were found as the main concentrated components of NP essential oil. All the doses of NP showed antinociception. NP 200 mg/kg reduced the pain sensation in tail flick (p <0.01) and formalin test (p <0.001 in both phases). In paw edema test, NP 100 and 200 mg/kg significantly reduced the inflammation (p <0.01 and p <0.05).ConclusionThis study reveals that the essential oil of NP may minimize both the acute and chronic forms of nociception and may have potent role against inflammation, but the dose should be maintained precisely to obtain the intended effect. |
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Keywords: | Nociception Nepeta pogonosperma Formalin test Tail flick test Essential oil Inflammation |
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