Protective effect of Pisonia aculeata on thioacetamide induced hepatotoxicity in rats |
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Authors: | C Anbarasu B Rajkapoor KS Bhat John Giridharan A Arul Amuthan K Satish |
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Institution: | 1.Department of Pharmacology, Karpagam University, Eachanari Post-641 021, Coimbatore, Tamil Nadu, India;2.Department of Pharmacology, Dayananda Sagar College of Pharmacy, Bangalore-560 078, India;3.Strides Arcolab, Bangalore-560 076, India;4.Regulatory Affairs, Strides Arcolab, Bangalore-560 076, India |
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Abstract: | ObjectiveTo evaluate the protective effect of Pisonia aculeata (P. aculeata) on thioacetamide induced hepatotoxicity in rats.MethodsMale Wistar rats were administered 250 or 500 mg/kg p.o. of P. aculeata extract for 21 days and simultaneously administered thioacetamide (TAA) 50 mg/kg bw s.c. 1 h after the respective assigned treatments every 72 h. At the end of all experimental methods, all the animals were sacrificed by cervical decapitation. Blood samples were collected. Serum was separated and analyzed for various biochemical parameters.ResultsTAA induced a significant rise in aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin, gamma glutamate transpeptidase (GGTP), lipid peroxidase (LPO) with a reduction of total protein, superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and glutathione S-transferase (GST). Treatment of rats with different doses of plant extract (250 and 500 mg/kg) significantly (P<0.001) altered serum marker enzymes and antioxidant levels to near normal against TAA treated rats. The activity of the extract at a dose of 300 mg/kg was comparable to the standard drug, silymarin (50 mg/kg, p.o.).ConclusionsIt can be concluded that P. aculeata extract possesses a remarkable hepatoprotective and antioxidant activity against TAA induced hepatotoxicity. More research is required to derive an optimal therapeutic dose. |
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Keywords: | Pisonia aculeate Thioacetamide Biochemical parameters Antioxidants Lipid peroxidation Histopathology Protective effect |
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