Evaluation of antimicrobial activity and bronchodialator effect of a polyherbal drug-Shrishadi |
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| Authors: | Divya Kumari Kajaria Mayank Gangwar Dharmendra Kumar Amit Kumar Sharma Ragini Tilak Gopal Nath Yamini Bhusan Tripathi JS Tripathi SK Tiwari |
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| Institution: | 1. Department of Diabetic Foot, Wolfson Medical Center, Israel;2. Department of Endocrinology, Wolfson Medical Center, Israel;3. Diabetes Unit, Wolfson Medical Center, Israel;4. Department of Medicine C, Wolfson Medical Center, Israel;5. Sackler Faculty of Medicine, Tel Aviv University, Israel;1. Breast Surgery Senior Fellow, Solihull Hospital, University Hospitals Birmingham Trust, Lode Lane, Solihull, Birmingham, B91 2JL, West Midlands, United Kingdom;2. Consultant Plastic and Reconstructive Surgeon, Plastic and Reconstructive Surgery Division, Good Hope Hospital, University Hospitals Birmingham Trust, Rectory Road, Sutton Coldfield, Birmingham, B75 7RR, West Midlands, United Kingdom;3. Senior Oncoplastic fellow, Plastic and Reconstructive Surgery Division, Good Hope Hospital, University Hospitals Birmingham Trust, Rectory Road, Sutton Coldfield, Birmingham, B75 7RR, West Midlands, United Kingdom;4. Plastic Surgery fellow, Plastic and Reconstructive Surgery Division, Good Hope Hospital, University Hospitals Birmingham Trust, Rectory Road, Sutton Coldfield, Birmingham, B75 7RR, West Midlands, United Kingdom;5. Senior Oncoplastic fellow, Plastic and Reconstructive Surgery Division, Good Hope Hospital, University Hospitals Birmingham Trust, Rectory Road, Sutton Coldfield, Birmingham, B75 7RR, West Midlands, United Kingdom;6. Consultant Radiologist, Department of Radiology, Good Hope Hospital, University Hospitals Birmingham, Rectory Road, Sutton Coldfield, Birmingham, B75 7RR, West Midlands, United Kingdom;7. Consultant Gynaecology & obstetrics, Department of Gynaecology & obstetrics, Good Hope Hospital, University Hospitals Birmingham Rectory Road, Sutton Coldfield, Birmingham, B75 7RR, West Midlands, United Kingdom;8. Consultant Thoracic Surgeon, Department of Thoracic Surgery, Heartlands Hospital, University Hospitals Birmingham, Bordesley Green East, Birmingham, B95SS, West Midlands, United Kingdom;9. Consultant Oncoplasty and Reconstructive Surgeon, Plastic and Reconstructive Surgery Division, Good Hope Hospital, University Hospitals Birmingham Trust, Rectory Road, Sutton Coldfield, Birmingham, B75 7RR, West Midlands, United Kingdom. |
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| Abstract: | ObjectiveTo investigate antimicrobial and bronchodialator effect of hydroalcholic extract of polyherbal drug Shirishadi containing Shirisha (Albezzia lebbeck), Nagarmotha (Cyprus rotandus) & Kantakari (Solanum xanthocarpum).MethodsAntimicrobial activity was evaluated by disc diffusion method and MIC, MBC, MFC were calculated by micro dilution method. Hydroalcholic extract of this preparation was investigated for its phytochemical analysis, phenol and flavonoid were determined by spectrophotometric method and in vivo bronchodilator effect was analysed by convulsion time.ResultsThe phytochemical tests revealed presence of alkaloids, anthraquinones, carbohydrates, flavonoids, saponins and tannins. The antimicrobial result showed the MIC of 6.25 mg/mL against Staphylococcus aureus and 12.5 mg/mL for Escherichia coli and 12.5 mg/mL against remaining bacteria tested, with strong antifungal activity. The maximum inhibition zone is found against Pseudomonas aeruginosa with MIC 16 mg/mL. Drug showed significant bronchodilator effect with 27.86% & 36.13% increase in preconvulsion time of guinea pigs pretreated with 100 & 200 mg/kg body weight of extract.ConclusionsThe study reveals that the extracts possess antibacterial activity and antifungal activity in a dose dependent manner. This antimicrobial property may be due to presence of several saponins, further studies are highly needed for the drug development. |
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| Keywords: | Shrishadi-polyherbal drug Phytochemical Phenolic & flavonoid content Candida species Bronchodilator |
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