Reduction of hydrogen peroxide-induced erythrocyte damage by Carica papaya leaf extract |
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Authors: | Tebekeme Okoko Diepreye Ere |
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Institution: | 1.Biochemistry Programme, Department of Chemical Sciences, Niger Delta University, PMB 71, Wilberforce Island, Bayelsa State, Nigeria;2.Pure and Applied Chemistry Programme, Department of Chemical Sciences, Niger Delta University, PMB 71, Wilberforce Island, Bayelsa State, Nigeria |
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Abstract: | ObjectiveTo investigate the in vitro antioxidant potential of Carica papaya (C. papaya) leaf extract and its effect on hydrogen peroxide-induced erythrocyte damage assessed by haemolysis and lipid peroxidation.MethodsHydroxyl radical scavenging activities, hydrogen ion scavenging activity, metal chelating activity, and the ferrous ion reducing ability were assessed as antioxidant indices. In the other experiment, human erythrocytes were treated with hydrogen peroxide to induce erythrocyte damage. The extract (at various concentrations) was subsequently incubated with the erythrocytes and later analysed for haemolysis and lipid peroxidation as indices for erythrocyte damage.ResultsPreliminary investigation of the extract showed that the leaf possessed significant antioxidant and free radical scavenging abilities using in vitro models in a concentration dependent manner (P<0.05). The extract also reduced hydrogen peroxide induced erythrocyte haemolysis and lipid peroxidation significantly when compared with ascorbic acid (P<0.05). The IC50 values were 7.33 mg/mL and 1.58 mg/mL for inhibition of haemolysis and lipid peroxidation, respectively. In all cases, ascorbic acid (the reference antioxidant) possessed higher activity than the extract.ConclusionsThe findings show that C. papaya leaves possess significant bioactive potential which is attributed to the phytochemicals which act in synergy. Thus, the leaves can be exploited for pharmaceutical and nutritional purposes. |
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Keywords: | Carica papaya Erythrocyte Haemolysis Lipid peroxidation Antioxidant Hydrogen peroxide Bioactive potential Free radical scavenging ability |
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