Alteration of chemical behavior of L-ascorbic acid in combination with nickel sulfate at different pH solutions in vitro |
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Authors: | Shaheen A Maniyar Jameel G Jargar Swastika N Das Salim A Dhundasi Kusal K Das |
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Institution: | 1.Environmental Health Research Unit, Department of Physiology, Al Ameen Medical College, Bijapur-586108 Karnataka, India;2.Department of Engineering Chemistry, B.L.D.E.A''s V.P.Dr.P.G. Halakatti College of Engineering and Technology, Bijapur-586103, Karnataka, India |
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Abstract: | ObjectiveTo evaluate the alteration of chemical behavior of L-ascorbic acid (vitamin C) with metal ion (nickel) at different pH solutions in vitro.MethodsSpectra of pure aqueous solution of L-ascorbic acid (E mark) compound and NiSO4 (H2O) (sigma USA) were evaluated by UV visible spectrophotometer. Spectral analysis of L-ascorbic acid and nickel at various pH (2.0, 7.0, 7.4 and 8.6) at room temperature of 29 °C was recorded. In this special analysis, combined solution of L-ascorbic acid and nickel sulfate at different pH was also recorded.ResultsThe result revealed that λmax (peak wavelength of spectra) of L-ascorbic acid at pH 2.0 was 289.0 nm whereas at neutral pH 7.0, λmax was 295.4 nm. In alkaline pH 8.6, λmax was 295.4 nm and at pH 7.4 the λmax of L-ascorbic acid remained the same as 295.4 nm. Nickel solution at acidic pH 2.0 was 394.5 nm, whereas at neutral pH 7.0 and pH 7.4 were the same as 394.5 nm. But at alkaline pH 8.6, λmax value of nickel sulfate became 392.0 nm. The combined solution of L-ascorbic acid and nickel sulfate (6 mg/mL each) at pH 2.0 showed 292.5 nm and 392.5 nm, respectively whereas at pH 7.0, L-ascorbic acid showed 296.5 nm and nickel sulfate showed 391.5 nm. At pH 7.4, L-ascorbic acid showed 297.0 nm and nickel sulfate showed 394.0 nm in the combined solution whereas at pH 8.6 (alkaline) L-ascorbic acid and nickel sulfate were showing 297.0 and 393.5 nm, respectively.ConclusionsResults clearly indicate an altered chemical behavior of L-ascorbic acid either alone or in combination with nickel sulfate in vitro at different pH. Perhaps oxidation of L-ascorbic acid to L-dehydro ascorbic acid via the free radical (HSc*) generation from the reaction of H2ASc + Ni (II) is the cause of such alteration of λmax value of L-ascorbic acid in the presence of metal nickel. |
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Keywords: | L-ascorbic acid Nickel pH Spectrum Chemical behavior Spectra UV visible spectrophotometer Peak wavelength Free radical Vitamin C Nickel sulfate |
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