大鼠脑挫伤后脑不同部位组织内钙含量的变化

背景神经细胞内钙超载引起一系列病理生化反应,从而对神经细胞造成损害.应用Ca2+通道阻滞剂药物治疗颅脑外伤为此提供了理论依据.目的探讨降低Ca2+-pO43-在电感耦合等离子体原子发射光谱(ICP-AES)法中的干扰,制定合适的分析条件,测定大鼠脑挫伤后脑组织中钙含量的变化.设计随机对照实验.地点和材料实验地点蚌埠医学院检验系医学化学实验室.选择体质量200～300g雄性wistar大鼠18只(由本院动物科提供),随机分为Ca2+对照组及外伤组,每组9只.干预实验不同浓度P(V)(0～2000 mg/L)在5个观测高度,对Ca2+谱线强度的影响;阳极电流改变时2 000 mg/LP(V)对Ca2+的影响;载气压力改变时P(V)对Ca2+的影响.用ICP-AES法检测脑挫伤后脑细胞内游离钙的浓度,并作病理学观测.主要观察指标脑挫伤后脑不同部位组织内的钙含量变化.结果本实验制定的分析条件可以明显降低pO43-的干扰,20次测定的相对标准偏差(RSD%)为1.16,样品加标回收率为97%.脑挫伤后脑细胞内游离钙明显增加.脑挫伤后,各脑区细胞内钙含量都明显高于对照组(t=3.45～46.39,P＜0.01).结论脑挫伤可能引起脑神经细胞膜上钙通道开放、钙离子大量内流,引起钙超载.优化ICP-AES实验条件对降低干扰提高分析结果的准确度至关重要.

Changes of the contents of cerebral calcium in different areas after cerebral contusion in rats

BACKGROUND: Intracellular calcium overload in neurocyte induces serials of pathophysiological reaction and therefore, causes injuries in neurocyte. The application of Ca2 + channel blocking agent in the treatment of craniocerebral trauma provides a theoretical gist.OBJECTIVE: To discuss the interference of Ca2 +-PO43 - in the measure of inductance coupling plasma atom emission spoctrum(ICP-AES) and to set proper analytical conditions for the assay of the changes of cerebral calcium content after cerebral contusion in rat.DESIGN: A randomized controlled study.SETTING and MATERIALS: The experiment was completed in Medical Chemical Laboratory of Analysis Department of Benghu Medical College. Eighteen male Wistar rats with a body weight from 200 g to 300 g obtained from animal department of our University were randomly allocated into Ca2 + control group and trauma group with 9 rats each.INTERVENTION: To study the impacts of different strength P(V) (0 -2 000 mg/L) at 5 observation heights on the intensity of Ca2+ spectrum; the impact of 2 000 mg/L of P(V) on Ca2 + when the current at positive pole changed; and the impact of P(V) on Ca2+ when air pressure changed. Cerebral intracellular free calcium strength after cerebral contusion was assayed by ICP-AES and the pathological changes were observed as well. ,MAIN OUTCOME MEASURES: The content changes of calcium at different cerebral areas after cerebral contusion.RESULTS: The analytical condition set in our study could significantly decrease the interference of PO43-, with the relative standard deviation(RSD% )of 1.16 for 20 times of assays and 97% of rate of recovery in marked samples. Cerebral intracellular free calcium significantly increased after cerebral contusion. Intracellular calcium contents at each cerebral area were significantly higher than that of control group( t = 3.45 - 46.39, P ＜ 0.01 ).CONCLUSION: Cerebral contusion could lead to the openness of calcium channel on the membrane of cerebral neurocyte, which would cause great intra-flow of calcium ions to induce calcium overload. It is extremely important to optimize the experimental conditions of ICP-AES for the decrease of the interference and the increase of the analytical accuracy.