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| 树突状细胞负载的肿瘤抗原肽疫苗抗脑胶质瘤作用的实验研究 | |
| 引用本文： | 赵丽波,李才,周维国.树突状细胞负载的肿瘤抗原肽疫苗抗脑胶质瘤作用的实验研究[J].中国实验诊断学,2009,13(1):21-23. |
| 作者姓名： | 赵丽波 李才 周维国 |
| 作者单位： | 1. 首都医科大学大兴医院肿瘤科,北京,102600;吉林大学药学院,吉林,长春,130012 2. 首都医科大学大兴医院肿瘤科,北京,102600 3. 吉林大学药学院,吉林,长春,130012 |
| 摘 要： | 目的探讨以树突状细胞（dendritic cells，DC）作为载体，将由脑胶质瘤细胞提取的热休克蛋白．肿瘤肽复合物（heat shock protein-tumor peptidecomplex，HSP-PC）负载到DC上所制备的肿瘤疫苗对脑胶质瘤细胞U251的杀伤作用。方法用脐带血在体外培养诱导DC，用脑胶质瘤患者术后标本提取肿瘤混合抗原并与DC共同培养，检测其对U251细胞的杀伤活性。结果经抗原负载后的DC对U251细胞的杀伤能力明显高于单纯DC对肿瘤细胞的杀伤能力。结论用肿瘤抗原负载的DC经体外实验证实具有明显的抗肿瘤效应，为临床肿瘤免疫治疗的开展提供了有效的数据。 |
| 关 键 词： | 树突状细胞 热休克蛋白-肿瘤肽复合物 免疫治疗 |
The Experiment on the Anti-spongiocytoma Effects of Dendritic Cells Tumor Vaccine |
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| ZHAO Li-bo,LI Cai,ZHOU Wei-guo.The Experiment on the Anti-spongiocytoma Effects of Dendritic Cells Tumor Vaccine[J].Chinese Journal of Laboratory Diagnosis,2009,13(1):21-23. | |
| Authors: | ZHAO Li-bo LI Cai ZHOU Wei-guo |
| Institution: | ZHAO Li-bo , LI Cai, ZHOU Wei-guo.( Pharmacy College of Medical University of Capital Auxiliary Tong Ren Hospital of BeiJing, 100000, China) . |
| Abstract: | Objective To study the execution of DCs tumor vaccine to spongiecytoma U251.DCs tumor vaccine were made by loading DC with heat shock protein-tumor peptide complex（HSP-PC） picked up from spongiocytoma cells. Methods We incubate DCs with HSP-PC picked up from spongiocytoma tissues in cord blood,then test its execution to U251 cells.Results The kill rate of DC loaded with HSP-PC to U251 cells were much higher than that of single DCs. Conclusion We found that Dendritic Cells Tumor Vaccine has significantly anti-spongiocytoma Effects,and this will give proof on cli/tical tumor mmunotherapy. |
| Keywords: | Dendritic cells Heat Shock Protein-Tumor PeptideComplex Immunothempy |
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