利奈唑胺在重症患者中药动学研究进展

目的 探讨不同提取工艺大黄外用对肺炎支原体（MP）感染小鼠JAK2/STAT3信号通路的影响，探究其改善小鼠肺部炎症的效应靶点。方法 将40只BALB/c小鼠随机分为正常组、模型组、生粉组、醇提组，每组10只。除正常组小鼠外，其余3组采用滴鼻法进行MP感染。造模后，将各组对应药物贴于小鼠背俞穴区30分钟/天，连续敷药7天。给药后第7天取材，光镜下观察小鼠肺组织、皮肤病理切片，计算肺组织病理评分、肺指数，采用ELISA法检测血清及肺组织中IL-6含量，运用Western Blot法及qPCR法检测STAT3、JAK2蛋白、mRNA的表达。结果 MP感染后肺泡间隔增厚，细支气管壁增厚，炎性细胞浸润，小鼠肺指数、肺组织炎症病理评分、血清及肺组织中IL-6含量、肺组织中STAT3和JAK2的蛋白表达及mRNA含量均升高（P＜0.05）。治疗后小鼠肺指数、肺组织炎症病理评分、血清及肺组织中IL-6含量均降低（P＜0.05），且醇提组作用效果较生粉组明显，但血清中含量差异不显著（P＞0.05）。与模型组比较，醇提组、生粉组STAT3、JAK2蛋白及mRNA含量下降（P＜0.05）。结论 大黄醇提物外用可有效改善MP感染小鼠肺部炎症，下调IL-6、JAK2及STAT3的表达，为其治疗肺炎支原体肺炎（MPP）提供了新的实验依据。

Research Progress on the Pharmacokinetics of Linezolid in Critically Ill Patients

Objective To investigate the effects of external application of Rhubarb in different extraction processes on JAK2/STAT3 signaling pathway in mice infected with Mycoplasma pneumoniae, and to explore the target of improving pulmonary inflammation in mice. Methods 40 BALB/c mice were randomly divided into normal group, model group, raw powder group and ethanol extract group, with 10 mice in each group. Except the normal mice, the other three groups were infected with MP by nasal dropping. After the model was established, the corresponding drugs of each group were applied to the Back-Shu acupoint area of mice for 30 min/day for 7 consecutive days. On the 7th day after administration, pathological sections of lung and skin of mice were observed under light microscopy. Lung histopathological scores and lung index were calculated. IL-6 content in serum and lung tissue was detected by ELISA. STAT3, JAK2 protein and mRNA expression were detected by Western Blot and qPCR. Results After MP infection, the alveolar septum, bronchiolar wall, inflammatory cell infiltration, lung index, inflammatory pathological score of lung tissue, IL-6 content in serum and lung tissue, the protein expression and mRNA content of STAT3 and JAK2 in lung tissue were all increased (P < 0.05). After treatment, lung index, pathological score of pulmonary inflammation, IL-6 content in serum and lung tissue of mice were decreased (P < 0.05), and the effect of alcohol extraction group was more obvious than that of raw powder group, but there was no significant difference in serum content (P > 0.05). Compared with model group, the contents of STAT3, JAK2 protein and mRNA in ethanol extract group and flour group decreased (P < 0.05). Conclusion External application of rhubarb alcohol extract can effectively improve the pulmonary inflammation in mice Infected with MP and down-regulate the expression of IL-6, JAK2 and STAT3, which provides a new experimental basis for its treatment of MPP.