小儿皮肤血管瘤c-myc蛋白表达与其细胞增殖和凋亡的关系

目的　探讨小儿皮肤血管瘤中c myc蛋白表达水平与血管瘤增生消退、细胞增殖活性及凋亡程度的关系。方法　以5 8例小儿皮肤血管瘤组织为研究对象 ,用免疫组织化学SP法分别检测c myc蛋白和增殖细胞核抗原的表达 ,并用末端脱氧核苷酸转移酶介导的末端标记技术做原位细胞凋亡检测。结果　 5 8例血管瘤组织中c myc蛋白表达率消退期显著高于增生期 (P <0 .0 1)。随着c myc蛋白表达强度增加 ,其细胞增殖活性相应降低 ,凋亡相应增加 ,c myc蛋白 ( )组、( )组分别与 (-)组、( )组比较 ,增殖指数和凋亡指数的差异均有显著性 (P <0 .0 1)。结论　小儿血管瘤内皮细胞c myc蛋白表达强度的增加可诱导内皮细胞凋亡 ,同时使细胞增殖受到相对抑制 ,提示c myc基因在血管瘤发生、发展和退化中起重要作用。

Relationship of c-myc Protein Expression with the Different Stage,Cell Proliferation and Apoptosis of Infants with Skin Hemangioma

Objective To investigate the relationship of c-myc protein expression in skin hemangioma of infant with different stage,cell proliferative activity and apoptos is.Methods Proliferating cell nudear antigen(PCNA) and c-myc were detected by immunohistoc hemical staining,and apoptosis was detected using in situ cell death detection( TUNEL method) in 58 skin hemangioma specimens of infant.Results Among the 58 hemangiomas,the positive rate of c-myc protein was significantly higher in involuting hemangiomas than that in proliferative hemangiomas(P< 0.01).With the increase in the expression of c-myc protein,the cell proliferat ing activity decreased and apoptosis increased.There was significant difference of cell proliferation and apoptosis between ( ) group and ( ) group,( ) gro up and (-) group,( ) group and ( ) group as well as ( ) group and (-) group( P<0.01).Conclusions Over-expression of c-myc protein can accelerate endothelial apoptosis and inhib it endothelial proliferation.c-myc gene may play an important role in the devel opment and involution of hemangiomas. J Appl Clin Pediatr,2005,20(2):147-148