| 坚杆火绒草质量标准的研究 |
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| 引用本文： | 赵雪莲,李奕松,苏宏娜,谭玲,李文兵,杨正明,刘圆. 坚杆火绒草质量标准的研究[J]. 中成药, 2022, 0(2): 380-384 |
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| 作者姓名： | 赵雪莲 李奕松 苏宏娜 谭玲 李文兵 杨正明 刘圆 |
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| 作者单位： | 西南民族大学药学院;四川省羌彝药用资源保护与利用技术工程实验室;青藏高原民族药用资源保护与利用国家民委重点实验室;西南民族大学青藏高原研究院;西南民族大学民族医药研究院 |
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| 基金项目： | 国家重点研发计划项目(2018YFC1708005);中央高校基本科研业务费专项资金研究类项目(2020NGD01);四川省科技计划项目(重点研发项目)(2021YFS0043)。 |
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| 摘 要： | 目的 建立坚杆火绒草质量标准.方法 显微法、TLC法进行定性鉴别,2020年版《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物含量,HPLC法测定绿原酸、3,5-O-二咖啡酰基奎宁酸含量.结果 显微特征明显,可见表皮细胞、气孔、非腺毛、梯纹导管、螺纹导管、木纤维、薄壁细胞、花粉粒等.TLC斑点清晰,分离度好.6批...
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| 关 键 词： | 坚杆火绒草 绿原酸 3 5-O-二咖啡酰基奎宁酸 显微法 TLC HPLC |
Quality standard for Leontopodium franchetii |
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| ZHAO Xue-lian,LI Yi-song,SU Hong-na,TAN Ling,LI Wen-bing,YANG Zheng-ming,LIU Yuan. Quality standard for Leontopodium franchetii[J]. Chinese Traditional Patent Medicine, 2022, 0(2): 380-384 |
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| Authors: | ZHAO Xue-lian LI Yi-song SU Hong-na TAN Ling LI Wen-bing YANG Zheng-ming LIU Yuan |
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| Affiliation: | (College of Pharmacy,Southwest Minzu University,Chengdu 610041,China;Sichuan Provincial Engineering Laboratory for Protection and Utilization Technology of Qiang-Yi Medicinal Resources,Chengdu 610225,China;National Ethnic Affairs Commission Key Laboratory for Protection and Utilization of Tibetan Plateau Ethnic Medicinal Resources,Chengdu 610225,China;Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610041,China;Institute of Ethnic Medicines,Southwest Minzu University,Chengdu 610041,China) |
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| Abstract: | AIM To establish the quality standard for Leontopodium franchetii Beauv..METHODS The qualitative identification was performed by microscopic method and TLC.The contents of water,total ash,acid-insoluble ash and extract were determined according to Chinese Pharmacopoeia(2020 edition) method.HPLC was adopted in the content determination of chlorogenic acid and 3,5-dicaffeoylquinic acid.RESULTS The microscopic features were obvious,epidermal cells,stomata,non-glandular hairs,glandular hairs,glandular scale,pitted vessel,threaded vessel,wood fibers,parenchyma cell and pollen grains could be observed.The TLC spots were clear with good resolution.The contents of water,total ash,acid-insoluble ash and extract were 0.92%-2.87%,7.28%-11.90%,1.08%-5.28% and 20.38%-23.02% in six batches of samples,respectively.Chlorogenic acid and 3,5-dicaffeoylquinic acid showed good linear relationships within their own ranges(R2=0.999 9),whose average recoveries were 99.22% and 98.96% with the RSDs of 1.51% and 1.46%,respectively.CONCLUSION This reasonable and feasible method can be used for the quality control of the L.franchetii. |
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| Keywords: | Leontopodium franchetii Beauv. chlorogenic acid 3,5-dicaffeoylquinic acid microscopic method TLC HPLC |
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