| NAIF1对胃癌细胞中IFIT家族蛋白的影响 |
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| 引用本文： | 张巧,黄常志,祝建疆,李燕,赵玫,李可心,李东东,戚红,黄声凯.NAIF1对胃癌细胞中IFIT家族蛋白的影响[J].肿瘤防治研究,2022,49(2):90-94. |
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| 作者姓名： | 张巧 黄常志 祝建疆 李燕 赵玫 李可心 李东东 戚红 黄声凯 |
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| 作者单位： | 1. 100089 北京，北京市海淀区妇幼保健院产前诊断中心；2. 100020 北京，国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院；3. 510000广州，中国科学院广州生物医药与健康研究院 |
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| 基金项目： | 国家自然科学基金(81902503);中国医学科学院医学与健康科技创新工程(2016-I2M-1-001)。 |
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| 摘 要： | 目的 探讨核诱导凋亡因子NAIF1在胃癌细胞系MKN45中的功能.方法 构建带有Tet-on系统的质粒pLVX-Tight-Flag-NAIF1-puro,在胃癌细胞系MKN45中,加入四环素衍生物强力霉素(Dox)诱导NAIF1表达,分别在加入Dox之后0、6、12、24 h收取细胞进行基因表达谱芯片检测,对差异表达...
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| 关 键 词： | 胃癌 MKN45 NAIF1 IFIT家族 |
| 收稿时间： | 2021-05-17 |
Effect of NAIF1 on IFIT Family Proteins in Gastric Cancer |
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| ZHANG Qiao,HUANG Changzhi,ZHU Jianjiang,LI Yan,ZHAO Mei,LI Kexin,LI Dongdong,QI Hong,HUANG Shengkai.Effect of NAIF1 on IFIT Family Proteins in Gastric Cancer[J].Cancer Research on Prevention and Treatment,2022,49(2):90-94. |
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| Authors: | ZHANG Qiao HUANG Changzhi ZHU Jianjiang LI Yan ZHAO Mei LI Kexin LI Dongdong QI Hong HUANG Shengkai |
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| Institution: | 1. Prenatal Diagnosis Center, Beijing Haidian Maternal and Child Health Hospital, Beijing 100089, China; 2. National Cancer Center/National Clinical Research Cencer for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical Colloge, Beijing 100020, China; 3. Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510000, China |
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| Abstract: | Objective To investigate the effect of NAIF1 in gastric cancer cell lines MKN45.Methods We constructed pLVX-Tight-Flag-NAIF1-puro plasmid with Tet-on system.DOX was added to induce NAIF1 expression in MKN45 cells.The cells were collected at 0,6,12 and 24 hours after DOX addition for gene expression microarray detection and biological analysis of differentially expressed genes.qRT-PCR and Western blot were used to verify the changes in mRNA and protein levels of the selected target differential genes.Results The biological analysis of gene microarray hybridization results showed that IFIT1,IFIT2 and IFIT3 expression significantly increased at 24h,qRT-PCR also showed this change,and Western blot further verified the change in protein level.However,IFIT5 showed no significant change in mRNA and gene expression.Conclusion Over-expression of NAIF1 in gastric cancer cells can promote the expression of some immune system-related IFIT family proteins. |
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| Keywords: | Gastric cancer MKN45 NAIF1 IFIT family |
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