滇桂艾纳香正丁醇部位HPLC指纹图谱建立及6种成分测定

目的建立滇桂艾纳香正丁醇部位HPLC指纹图谱,并测定原儿茶酸、绿原酸、咖啡酸、异绿原酸A、异绿原酸B、异绿原酸C含量。方法分析采用ZORBAX SB-C₁₈色谱柱(150 mm×4.6 mm,5μm);流动相乙腈-0.2%磷酸,梯度洗脱,建立HPLC指纹图谱,采用2012年版《中药色谱指纹图谱相似度评价系统》软件进行相似度评价,SPSS 23.0软件进行聚类分析及主成分数据分析,同时测定6种成分含量。结果 12批滇桂艾纳香有12个共有峰,各批次药材相似度均大于0.9。聚类分析结果显示12批滇桂艾纳香药材可分为2类,主成分分析表明,前2个成分的累计方差贡献率为96.689%。6种成分在其各自的范围内线性关系良好(r≥0.999 0),精密度、重复性、稳定性的RSD值均小于3%,加样回收率平均值为97.1%~102.7%,RSD为1.81%~2.82%。结论该方法稳定准确,可为滇桂艾纳香正丁醇活性部位质量评价提供参考依据。

Establishment of HPLC fingerprints and determination of six constituents for n-butanol fraction of Blumea riparia

AIM To establish the HPLC fingerprints of the n-butanol fraction of Blumea riparia(Bl.) DC,and to determine the contents of protocatechuic acid,chlorogenic acid,caffeic acid,isochlorogenic acid A,isochlorogenic acid B and isochlorogenic acid C.METHODS The test was conducted on a chromatographic column of ZORBAX SB-C₁₈(150 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2% phosphoric acid aqueous solution for gradient elution.The software of Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2012 Edition) and SPSS 23.0 were used for the establishment of the HPLC fingerprints and similarity evaluation,and the simultaneous determination of the contents of six active components.RESULTS Among the twelve common peaks shared by 12 batches of B.riparia,more than 0.9 similarities in the constituents contained inside the medicinal materials were observed.The results of cluster analysis revealed the 2 major categories among the 12 batches of B.riparia.Principal component analysis data showed that the first two components contributing 96.689% cumulative variance rate.Among the 6 constituents,each had a good linear relationship(r≥0.999 0) in its own range;less than 3% RSD values of precision,repeatability and stability,with 97.1%-102.7% average recovery rate and 1.81%-2.82% RSD.CONCLUSION This stable and accurate method provides reference for the quality evaluation of n-butanol active site of B.riparia.