香青兰总黄酮对心肌缺血再灌注损伤大鼠氧化应激和线粒体的保护作用

目的 研究香青兰总黄酮对心肌缺血再灌注损伤(MIRI)过程中氧化应激和线粒体损伤的影响.方法 结扎大鼠冠状动脉左前降支缺血30 min,再灌注灌2 h构建MIRI模型.SD大鼠随机分为假手术组、模型组、香青兰总黄酮组、香青兰总黄酮+AMPK抑制剂Compound C组、香青兰总黄酮+SIRT1抑制剂EX-527组,每组...

Protective effects of Dracocephalum moldavica total flavonoids on oxidative stress and mitochondria in rats with myocardial ischemia-reperfusion injury

AIM To study the effects of Dracocephalum moldavica L.total flavonoids on oxidative stress and mitochondrial damage during myocardial ischemia-reperfusion injury(MIRI).METHODS The MIRI rat models were established by 2 hours of reperfusion following 30 min coronary occlusion.SD rats were randomly divided into sham group,model group,D.moldavica total flavonoids group,the group for use of D.moldavica total flavonoids and AMPK inhibitor Compound C,and the group for use of D.moldavica total flavonoids and SIRT1 inhibitor EX-527,with 10 rats in each group.The rats were subsequently subjected to the determination of myocardial infarction area by TTC staining;the measurement of antioxidant capacity in terms of ROS and MDA levels,and SOD activity;the investigation of the protein expressions of AMPK,SIRT1 and PGC-1α by immunohistochemistry,and the mitochondrial ultrastructure by transmission electron microscopy.RESULTS Compared with the model group,the group intervened solely with D.moldavica total flavonoids shared reduced myocardial infarction area(P<0.01) and ROS and MDA levels(P<0.01),increased SOD activity(P<0.01),increased protein expressions of AMPK,SIRT1 and PGC-1α(P<0.05,P<0.01),and improved mitochondrial ultrastructure damage.Compared with D.moldavica total flavonoids group,the groups given combinative administration with either Compound C or EX-527 displayed inferiority in terms of the aforementioned indices levels(P<0.05,P<0.01).CONCLUSION Being an MIRI inhibitor,D.moldavica total flavonoids may activate AMPK/SIRT1/PGC-1α signal transduction pathway.