血小板促进肺癌细胞A549增殖与迁移的体外实验研究

目的探讨外周血血小板对肺腺癌A549细胞增殖和迁移力的影响。方法将从健康人外周血中分离到的血小板与A549细胞共培养,采用CCK-8实验检测A549细胞的增殖情况,Transwell实验检测细胞的迁移能力。结果共培养1 h,镜下可见血小板向瘤细胞聚集。血小板可增强A549细胞的增殖力,并存在一定的剂量依赖性,共培养时间对瘤细胞增殖力的影响与血小板的加入量有关(P<0.05)。与血小板共培养的A549细胞数穿透Transwelll小室基底膜的数量(128.6±16.0)明显多于A549单独培养组(84.4±12.0),差异有统计学意义(P<0.05)。结论血小板可诱导肺腺癌A549细胞的增殖和迁移,血小板有望成为治疗肺癌的靶点。

Effect of platelet on the proliferation and migration of lung cancer A549 cells:an in vitro experimental study

Objective To explore the effect of peripheral blood platelets on the proliferation and migration of A549 cells.Methods Platelets isolated from healthy people peripheral blood were co-cultured with A549 cells.The proliferation of A549 cell was detected by Cell Counting Kit-8(CCK-8)assay,and cell migration ability was detected by Transwell assay.Results After 1 hour of co-culture,platelet aggregated towards tumor cells was observed under light microscopes.The results of CCK-8 assay showed that platelets could enhance the proliferation ability of A549 cells in a dose-dependent manner.The effect of co-culture time on the proliferation of A549 cells was related to the amounts of platelets added(P<0.05).The Transwell results showed that the number of A549 cells penetrating the artificial basement membrane in the co-culture system(128.6±16.0)was significantly higher than that of A549 cultured alone(84.4±12.0),and the difference was statistically significant(P<0.05).Conclusion Platelets can induce the proliferation and migration of A549 cells,indicating that platelets are expected to become therapeutic target for lung cancer treatment.