藏花素调控deltaNP63基因抑制膀胱肿瘤细胞生长的研究

目的探讨藏花素对膀胱肿瘤细胞5637的生长抑制作用及调控机制。方法用3mmol／L藏花素处理膀胱肿瘤细胞5637细胞株,采用反转录PCR（RT-PCR）检测细胞deltaN P63基因表达的变化,光镜下检测细胞形态改变,流式细胞术检测细胞周期改变,四甲基偶氮唑盐法（MTT）检测细胞增殖改变。结果在一定浓度的藏花素作用下,可见5637细胞生长缓慢,deltaN—P63基因表达下降,G0／G1期细胞数量增加,G2／M期细胞数量减少,MTT检测显示细胞增殖受到抑制。结论藏花素可以抑制膀胱肿瘤细胞增殖和生长,其机制可能与通过下调deltaN P63基因的表达有关。

Crocin inhibits bladder cell growth through regulation of deltaN P63 gene

Objective To detect the inhibition effects and possible mechanism of crocin in bladder cancer 5637 cell line. Methods 5637 cells were treated with 3 mmol/L crocin,and the change of deltaN P63 gene was detected using reverse transcription-polymerase chain reaction （RT-PCR） ,the cell form was observed by microscope, cell cycle was detected by flow cytometry, and cell pro- liferation was detected by MTT. Results In the optimal concentration of crocin,the growth of 5637 cells became slowly than nor- mal. The expression of deltaN P63 gene decreased, the cell number of G0/G1 was increased but the cell number of Gz/M decreased. MTT result showed that the cell proliferation was inhibited. Conclusion Bladder cancer cell growth and proliferation could be in- hibited by crocin,and its possible mechanism is the down regulation of deltaN P63 gene.