脑膜瘤血管内皮细胞生长因子与瘤周脑水肿关系的研究

目的 了解血管内皮细胞生长因子（ＶＥＧＦ）在脑膜瘤中的表达,探讨ＶＥＧＦ与瘤周脑水肿和微血管形成的关系。方法 应用免疫组化和免疫印迹技术,检测３３例脑膜瘤组织中ＶＥＧＦ蛋白表达与瘤周脑水肿和微血管形成的关系。结果 脑膜瘤ＶＥＧＦ表达率７５％,４例可疑表达。ＶＥＧＦ蛋白表达与ＰＶＢＥ和微血管形成存在肯定的相关产地脑膜瘤组织中,在瘤周脑水肿和微血管形成方面发挥重要作用。

Vascular endothelial factor of meningomas and peritumoural vasogenic brain edema

OBJECTIVE: To study the expression of vascular endothelial growth factor (VEGF) in meningomas correlated with peritumoral vasogenic brain edema (PVBE) and angiogenesis. METHODS: VEGF protein expression in 33 patients with meningomas (12 men, average 50 +/- 12 years; 21 women average 46 +/- 14 years) was examined by immunohistochemical and western blotting. The number of microvessels which were immunostained with von willelrand factor was counted and PVBE was assessed blindly before MRI scan or CT scan. RESULTS: The expression of VEGF protein was found in 75.8% (25/33). The mean rate of immunostaining was 2.03 and the mean rate of PVBE was 2.39. The rate of staining intensity was correlated with PVBE rate (r = 0.7352, P < 0.01) and vascular counts (r = 0.6974, P < 0.01). VEGF protein isoform of molecular sizes corresponding to VEGF165 was detected in cytosolic extracts of meningomas. CONCLUSIONS: VEGF is highly related to PVBE and angiogenesis of meningoma. Wide-spread distribution of VEGF in meningomas suggests VEGF165 isoform.