| 人乳头瘤病毒16型晚期蛋白在昆虫细胞中的表达 |
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| 引用本文： | 魏兰兰, 韩立群, 谷鸿喜, 凌秋. 人乳头瘤病毒16型晚期蛋白在昆虫细胞中的表达[J]. 中国公共卫生, 2002, 18(6): 697-698. DOI: 10.11847/zgggws2002-18-06-29 |
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| 作者姓名： | 魏兰兰 韩立群 谷鸿喜 凌秋 |
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| 作者单位： | 1.哈尔滨医科大学微生物学教研室 哈尔滨150086;2.中国预防医学科学院遗传与免疫室;3.哈尔滨医科大学动物学部 |
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| 基金项目： | 黑龙江省科委课题(G98-C0803) |
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| 摘 要： | 目的 构建HPV16型晚期蛋白重组杆状病毒,并使其在昆虫细胞中获得高效表达.方法 首先用线性化的杆状病毒DNA与重组杆状病毒转移质粒共转染sf9昆虫细胞进行同源重组,获得2株重组杆状病毒.结果 经鉴定该重组病毒中有目的DNA存在且可表达晚期蛋白;电镜观察发现重组杆状病毒感染的sf9细胞核内有乳头瘤病毒VLPs.结论 HPV16型晚期蛋白在昆虫细胞中的成功表达为HPV16型预防性基因工程亚单位疫苗的研制和诊断试剂的研究开发奠定了基础.
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| 关 键 词： | 人乳头瘤病毒16型 病毒样颗粒 重组杆状病毒 |
| 文章编号： | 1001-0580(2002)06-0697-02 |
| 收稿时间： | 2001-10-23 |
| 修稿时间： | 2001-10-23 |
Expression of Human Papillomavirus Type 16 Capsid Proteins in Baculovirus-insect Cell System |
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| WEI Lan-lan, HAN Li-qun, GU Hong-xi, . Expression of Human Papillomavirus Type 16 Capsid Proteins in Baculovirus-insect Cell System[J]. Chinese Journal of Public Health, 2002, 18(6): 697-698. DOI: 10.11847/zgggws2002-18-06-29 |
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| Authors: | WEI Lan-lan HAN Li-qun GU Hong-xi |
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| Affiliation: | 1.Department of Microbiology Harbin Medical University, Harbin 150086, China |
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| Abstract: | Objective To construct the recombinant baculoviruses containing L1 or L1 Plus L2 genes and express high-efficiency capsid proteins in insect cells.Methods By co-transfection of linearized baculovirus DNA and recombinant baculovirus transfer plasmids containing L1 or L1+L2 respectively,two recombinant baculovir us strains were gotten.Results The L1 and L2 genes of capsid protein were amplified by PCR in recombinant baculovirus;The 57KD L1 protein and the 95 KD,110KD L2 proteins were detected by Coomassie-blue staining and Western-blot;ME studies showed that there were the HPV16 virion in sf9 cells.Conclusion These results would be the basis to develop the HPV 16 prophylactic vaccines and diagnosis kit. |
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| Keywords: | human papillomavirus type 16 VLPs recombinant baculovirus |
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