宁夏栽培秦艽与野生秦艽有效成分的比较

目的:通过对宁夏栽培秦艽不同品种与野生秦艽有效成分的比较,评价宁夏栽培秦艽的质量.方法:采用《中国药典》2010年版一部方法测量龙胆苦苷和马钱苷酸的含量;采用全自动氨基酸分析仪测定其氨基酸的含量;采用蒽酮-硫酸法测定其多糖的含量.结果:宁夏栽培的3种秦艽龙胆苦苷、马钱甘酸的含量都高于《中国药典》规定的限度;与野生秦艽相比,所含氨基酸的含量有显著性差异;多糖的含量无显著性差异.结论:宁夏栽培的3种秦艽品质良好,对于秦艽氨基酸和多糖的药用价值,可以进一步的研究开发和利用.

Comparative of Effective Components from Cultivated and Wild Gentiana macrophylla in Ningxia Province

Objective: To evaluate the quality of cultivated Gentiana in Ningxia Province by the comparative study of effective components in different varieties and wild Gentiana. Method: The content of gentiopicroside and loganin acid was determined according to the method listed in 2010 version of Chinese Pharmacopoeia. The amino acid content was determined by automatic amino acid analyzer, and the content of polysaccharide was determined by anthrone-sulfuric acid method. Result: The content of gentiopicroside and loganin acid in three varieties of Gentiana cultivated in Ningxia was higher than prescribed limits in Chinese Pharmacopoeia, and there were no significant differences in amino acid content, and there were significant differences in the content of polysaccharides compared with wild Gentiana. Conclusion: The quality of three kinds of Gentiana macrophylla cultivated in Ningxia is good. The medicinal value of amino acids and polysaccharides in Gentiana macrophylla can be further researched and utilized.