A rapid hemi-nested PCR for HTLV-I detection. |
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| Authors: | Davide Gibellini Paola Monari Monica Sassi Maria Carla Re |
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| Affiliation: | Section of Microbiology, Department of Clinical and Experimental Medicine, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy. |
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| Abstract: | A hemi-nested PCR approach was adopted to detect HTLV-1 infection in clinical samples of peripheral blood mononuclear cells (PBMCs) from subjects with positive or indeterminate serological results. Our results showed that the hemi-nested PCR quickly solved the diagnostic query, detecting the presence of proviral HTLV-1 DNA in two of the 252 patients with inconclusive serological results. The main advantage of this method are the typology of DNA extraction, allowing a consistent DNA recovery without amplification problems, the rapidity (4-5 hours), the performance of the assay and its comparable or better sensitivity than other HTLV-1 PCR formats. |
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