Radioimmunotherapy of human glioma xenografts in nude mice by indium-111 labelled internalising monoclonal antibody |
| |
Authors: | Saga T Sakahara H Nakamoto Y Sato N Zhao S Aoki T Miyatake S Namba Y Konishi J |
| |
Affiliation: | Department of Nuclear Medicine, Faculty of Medicine, Kyoto, Japan. saga@kuhp.kyoto-u.ac.jp |
| |
Abstract: | The potential of 111Indium (111In)-labelled internalising anti-integrin alpha 3 antibody GA17 in the radioimmunotherapy of human glioblastoma xenografts in nude mice was investigated. A radioisotope retention assay showed a rapid release of radioiodine from the glioblastoma cells after the binding of 125I-GA17, whilst 111In-GA17 was retained in the cells for a longer time period. The glioblastoma xenografts showed a high and prolonged uptake of 111In-GA17, and tumour uptake of 125I-GA17 was lower and decreased with time. In the mice which received two injections of 18.5 MBq of 111In-GA17, the growth of the subcutaneous tumour was significantly suppressed compared with the untreated group and mice injected with an 111In-labelled control antibody. These results indicate that GA17 was internalized into the glioblastoma cells and that 111In was retained within the cancer cells. The injection of a high-dose of 111In-GA17 can suppress the growth of tumour xenografts in nude mice. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |