syndecan-1在糖尿病视网膜中的表达

目的:观察syndecan-1在增殖性糖尿病视网膜病变患者视网膜增殖膜及糖尿病大鼠视网膜中的表达变化。方法:SD大鼠行链脲佐菌素腹腔注射以诱导糖尿病。造模成功后,墨汁灌注及视网膜铺片观察视网膜的血管情况。免疫组织化学染色检测大鼠视网膜及人糖尿病视网膜增殖膜中syndecan-1蛋白的表达。结果:糖尿病大鼠9wk时,视网膜周边血管走形迂曲,毛细血管网明显减少,部分毛细血管灌注不良。对照组大鼠的视网膜syndecan-1呈阳性表达,其中神经纤维层、节细胞层呈强阳性表达,内丛状层和光感受器外节呈中度阳性表达,外丛状层呈弱阳性表达。糖尿病大鼠视网膜中,syndecan-1的表达减低,其中神经纤维层、神经节细胞层、光感受器外节呈中度阳性表达,内丛状层及外丛状层呈弱阳性表达。13例人糖尿病视网膜病变视网膜增殖膜标本中,syndecan-1弱阳性表达8例(61.5%),阴性表达5例(38.5%)。结论:临床和动物实验共同表明,糖尿病状态下,视网膜中syndecan-1的表达降低。

Expression of syndecan-1 in the retina of diabetes

AIM:To observe the expression of syndecan-1 on epiretinal membranes obtained from patients with proliferative diabetic retinopathy and retina of diabetic rats.

METHODS:SD rats were given intraperitoneal injection of streptozotocin to induce diabetes. Ink perfusion and stretched retina were used to observe the retinal blood vessels. Immunohistochemistry staining was used to detect syndecan-1 protein in the rat retina and epiretinal membranes of proliferative diabetic retinopathy.

RESULTS:In the diabetic rats of 9-week, peripheral retinal blood vessels shaped tortuously, capillary network was significantly reduced, and parts of the capillary perfusion was bad. In the control group, strong positive staining of syndecan-1 was detected in the nerve fiber layer and ganglion cells, moderate positive staining was observed in the inner plexiform layer and outer segments of photoreceptor, and weak positive staining was detected in the outer plexiform layer. In the retina of diabetic rats, syndecan-1 decreased. Moderate positive staining of syndecan-1 was observed in the nerve fiber layer, ganglion cells and outer segments of photoreceptor, weak positive staining was detected in the inner plexiform layer and outer plexiform layer. Among 13 specimens of epiretinal membranes, weak positive staining of syndecan-1 was detected in 8 cases(61.5%)and negative staining was observed in 5 cases(38.5%).

CONCLUSION:Syndecan-1 is down-regulated in the retina of diabetic rats and epiretinal membranes of proliferative diabetic retinopathy.