氯喹逆转人鼻咽癌细胞HNE1/DDP的耐药作用

目的探究氯喹对人鼻咽耐药细胞的逆转耐药作用及其机制。方法MTT检测不同浓度的顺铂（2, 4, 8, 16, 32 μmol·L-1）
以及不同浓度的氯喹（5, 10, 20, 40, 80 μmol·L-1）处理HNE1细胞，HNE1/DDP细胞48 h 对细胞增殖的影响；q-PCR方法检测5，
10 μmol·L-1 氯喹处理HNE1/DDP后的多药耐药基因MDR1 mRNA的表达；PI单染方法检测氯喹（10, 20 μmol·L-1）处理HNE1/
DDP后细胞凋亡率；采用Western blot 方法检测氯喹处理HNE1/DDP后的P-糖蛋白（P-glycoprotein, P-gp）的表达。结果MTT
结果显示，不同浓度的氯喹（5, 10, 20, 40, 80 μmol·L-1）对细胞具有明显的抑制作用，并且呈浓度依赖性；q-PCR结果表明，氯喹
作用细胞后能明显降低MDR1 mRNA水平；PI 结果显示，氯喹作用细胞后，细胞的凋亡率明显增加；通过western blot 实验表
明，氯喹能明显降低MDR1和P-gp 蛋白水平。结论氯喹能逆转人鼻咽癌细胞HNE1/DDP的耐药，其机制可能与下调MDR1
mRNA表达及抑制P-gp的功能与表达有关。

Effects of chloroquine in reversing multidrug resistance in HNE1/DDP cell line

Objective To investigate the effect of chloroquine in reversing multidrug resistance (MDR) of HNE1/DDP cell line
and explore the mechanism. Methods MTT assay was used to detect the cell viability of HNE1 and HNE1/DDP after
exposure to different concentrations of DDP (2, 4, 8, 16 and 32 μmol/L) and different concentrations of chloroquine (5, 10, 20,
40, and 80 μmol/L). q-PCR was used to assess the expression of MDR1 mRNA and Western blotting was employed to detect
P-glycoprotein (P-gp) expression in HNE1 and HNE1/DDP cells exposed to 5 and 10 μmol/L chloroquine. The cell apoptosis
rate of HNE1 and HNE1/DDP cells exposed to 10 and 20 μmol/L chloroquine was determined by PI assay. Results
Chloroquine exposure caused dose-dependent suppression of the proliferation in both HNE1 and HNE1/DDP cells, and
significantly reversed multidrug resistance in HNE1/DDP cells. The expressions of MDR1 mRNA and P-gp protein were
significantly lowered in the cells treated with chloroquine. Conclusion Chloroquine can reverse multidrug resistance in HNE1/
DDP cells possibly through down-regulation of MDR1 and inhibition of P-gp protein.