电针足阳明经穴对胃粘膜损伤大鼠胃粘膜血流量及生长抑素的影响

目的 :观察电针足阳明经“四白”、“天枢”、“足三里”穴对胃粘膜损伤大鼠胃窦和延髓内生长抑素(SS)含量的影响及与胃粘膜损伤、胃粘膜血流量的关系 ,以探讨经脉 -脏腑相关的物质基础。方法 :健康 SD大鼠 6 0只 ,随机分为正常对照组 (对照组 ) ,模型组 ,针刺四白组、天枢组及非穴点组共 6组 ,以乙醇灌胃造成胃粘膜损伤大鼠模型 ,观察电针大鼠足三里、天枢、四白穴对胃粘膜血流量 (氢气清除法 )的影响 ,用放免分析法检测大鼠胃窦及延髓 SS含量。结果 :胃粘膜血流量在胃粘膜损伤后明显降低 ,针刺四白、天枢、足三里及非穴点后 ,均有不同程度升高 ,尤以足三里和四白组升高明显 (P<0 .0 1) ;胃窦及延髓 SS模型组较对照组升高 (P<0 .0 5 ,<0 .0 1) ,而针刺四白、足三里组升高不明显 (P>0 .0 5 )。结论 :胃窦、延髓 SS含量变化与胃粘膜血流量的改变有一定关系 ,电针足阳明经四白、足三里穴可能通过对胃窦及延髓 SS含量的改变来影响胃粘膜血流量 ,促进胃粘膜损伤的修复。

Effect of Stimulating Points of the Stomach Meridian of Foot-yangming by Electronic Needle on Gastric Mucosal Blood Flow and Somatostatin in the Rat Model of Gastric Mucosal Injury

Objective: To observe the changes of somatostatin (SS) in gastric antrum (GA) and medulla oblongata (MO) respectively, gastric lesion index and gastric mucosal blood flow (GMBF) in rat model of gastric mucosal injury when Sibai, Tianshu and Zusanli points of the Stomach Meridianof Foot- yangming were stimulated with electronic acupuncturing( EA) ,and to explore the material base for the relationship between meridian and viscera.Methods:Sixty SD rats were randomly divid- ed into six groups:acupuncturing- Zusanli- Tianshu- Sibai group,normal group,model group, acupuncturing- Sibai group,acupuncturing- Tianshu group and acupuncturing non- point group.Gas- tric mucosalinjury model was made by filling ethanol into stomach. GMBF was detected with hydro- gen- cleaning and SS in gastric anturm and medulla oblongata measured with radioimmunoassay ( RIA) . Results:GMBF decreased obviously after gastric mucous membrane injury. After acupunc- turing Sibai,Zusanli,Tianshu and nonpoint,GMBF increased in different degree.The increase of the acupuncturing- Zusanligroup and acupuncturing- Sibaigroup was moreobvious( P <0 .0 1 ) .SSin GA and MO of gastric mucosa injury group washigherthan thatof normalgroup( P <0 .0 5 or P < 0 .0 1 ) .The increase in both acupuncturing Sibai group and Zusanli group was not obvious( P >0 . 0 5 ) . Conclusion:SSin GA and MO is related with GMBF. Stimulating Sibaiand Zusanliby EA can accelerate recovery of gastric mucosa injury,which may change the content of SS in GA and MO to affect GMBF.