紫杉醇对子宫癌患者血清及组织相关因子的影响

目的：探讨紫杉醇对子宫癌患者血清及组织缺氧诱导因子-1α（ HIF-1α）、血管内皮生长因子（ VEGF）等相关因子的影响。方法回顾性分析48例子宫癌患者的临床资料，采用紫杉醇联合化疗方案对其进行治疗，在治疗前后，采用elisa法检测患者血清中HIF-1α和VEGF的含量，采用免疫组织化学法对患者的宫颈组织进行染色，观察HIF-1α和VEGF的表达情况，western bloting法检测宫颈组织中HIF-1α和VEGF表达水平的变化，并比较发生淋巴转移和未发生淋巴转移患者的HIF-1α和VEGF表达的改变。结果未发生淋巴转移子宫癌患者血清中HIF-1α和VEGF的含量分别为（16．71±34．75）ng/L、（255．77±87．99）ng/L，治疗后分别为（98．33±32．17）ng/L、（153．67±65．45）ng/L，治疗前后差异均有统计学意义（t＝9．416、5．001， P＝0．003、0．007）；发生淋巴转移的子宫癌患者血清中HIF-1α和VEGF的含量升高，与未发生转移患者比较差异均有统计学意义（ t＝3．055、16．196，P＝0．038、0．000）；治疗后HIF-1α和VEGF含量下降；宫颈癌患者在接受治疗后，宫颈组织HIF-1α和VEGF的表达水平均明显下降，与治疗前比较差异有统计学意义（ t＝5．899， P＝0．004；t＝9．649，P＝0．001）；发生淋巴转移的患者在接受治疗后，HIF-1α的表达水平下降，与治疗前比较差异有统计学意义（t＝5．233，P＝0．004），但VEGF的表达未见明显变化。结论紫杉醇抗子宫癌的作用机制可能与抑制HIF-1α与VEGF蛋白的表达有关，进而抑制肿瘤组织血管生成和癌细胞转移。

Influence of paclitaxel on the correlation factors in serum and tissue of patients with metrocarcinoma

Objective To explore the level of hypoxia-inducible factor-1α（ HIF-1α） and vascular endotheli-al growth factor（ VEGF） in serum and tissue of patients with metrocarcinoma.Methods 48 patients diagnosed with cervical cancer were analyzed retrospectively.Patients were treated with paclitaxel combination chemotherapy,HIF-1αand VEGF levels in serum of patients were detected by ELISA method before and after the treatment.HIF-1αand VEGF levels in cervical tissue of patients were observed by immunehistochemical staining methods.Western blot method was used to detect the change of HIF-1αand VEGF expression levels in cervical tissues of patients.And the levels of HIF-1αand VEGF in patients with lymphatic metastasis and lymph node metastases were compared. Results Lymph node metastasis did not occur in patients with uterine cancer serum HIF-1αand VEGF were （16.71 ±34.75）ng/L,（255.77 ±87.99）ng/L,respectively,and those after treatment were （98.33 ±32.17）ng/L, （153.67 ±65.45）ng/L,the differences were statistically significant（t=9.416,5.001,P=0.003,0.007） before and after treatment;The serum of patients with lymph node metastasis of uterine cancer in HIF-1αand VEGF increased, and cervical cancer patients after treatment,cervical tissue HIF-1αand VEGF;The metastasis were statistically signif-icant differences occurred（t=3.055,16.196,P=0.038,0.000）;HIF-1αand VEGF decreased after treatment,the expression levels were significantly decreased compared with before treatment （ t=5.899,P=0.004;t=9.649,P=0.001）;patients with lymph node metastasis after treatment,the expression levels of HIF-1αdecreased compared with before treatmen （ t =5.233, P =0.004）, but there was no significant changes in the expression of VEGF. Conclusion The mechanism of taxol anti-uterine cancer may be related with inhibition the expression of HIF-1αand VEGF protein,and thereby inhibiting tumor angiogenesis and cancer metastasis.