冻融优质胚胎体外培养时间对临床结局的影响

目的:探讨玻璃化冷冻复苏后优质胚胎的培养时间对临床结局的影响。方法:采用回顾性研究方法,将2011~2012年在本院实施冻融胚胎移植(FET)的1061例分为3组,其中冷冻胚胎复苏后的优质胚胎培养2~4h移植为A组(96例);培养4~8h移植为B组(615例);培养8h移植为C组(350例),比较各组胚胎植入率、临床妊娠率、流产率及活产率。结果:3组患者年龄、不孕年限、体重指数、移植日子宫内膜厚度、移植胚胎个数无统计学差异(P0.05);胚胎植入率(24.7%、23.4%、28.2%)、临床妊娠率(43.8%、40.8%、44.9%)、早期流产率(4.8%、10.4%、12.1%)、活产率(40.6%、32.7%、37.4%)均无统计学差异(P均0.05)。结论:玻璃化冷冻胚胎复苏后培养时间对FET周期临床结局无明显影响,可根据临床和患者情况,具体安排胚胎移植时间。

Effect of culture time on clinical outcomes in vitrification frozen-thawed embryos transfer cycles

Objective： To explore the influence of the duration of embryo culture after thawing on clinical pregnancy outcomes in frozen-thawed embryos transfer cycles. Methods： A retrospective analysis was performed on 1061 frozen- thawed embryo transfer cycles, which were divided into three groups according to different cultures times, namely, group A （2-4 hours after thawing, n~96） , group B （〉4-8 hours after thawing, n=615） and group C （〉8 hours af- ter thawing, n=350）. The clinical pregnancy rate, embryo implantation rate, abortion rate and live birth rate among groups were compared. Results： There were no significant difference in age, duration of infertility, body mass index, endometrial thickness on the embryo transplant day, the number of embryo transferred among the three groups. There were no significant differences among the three groups in the implantation rate （24.7% vs. 23.4% vs. 28.2%, P〉 0.05）, clinical pregnancy rate （43.8% vs. 40.8% vs. 44.9%, P〉0.05）, early abortion rate （4.8% vs. 10.4% vs. 12.1%, P〉0.05） and live birth rate （40.6% vs. 32.7% vs. 37.4%, P〉0.05）. Conclusion： The incubation time in vitro of frozen-thawed embryos may not significantly affect the pregnancy outcomes, and the embryo transplant time could be chosen according to clinical and patients＂ conditions.