| 熟地含药血清对骨髓间质干细胞向神经细胞分化的影响 |
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| 引用本文： | 黄勇,吴建军,刘凯,刘永琦,陈丽,孙少伯.熟地含药血清对骨髓间质干细胞向神经细胞分化的影响[J].中国老年学杂志,2011,31(20). |
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| 作者姓名： | 黄勇 吴建军 刘凯 刘永琦 陈丽 孙少伯 |
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| 作者单位： | 甘肃中医学院,甘肃兰州,730000 |
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| 摘 要： | 目的 观察熟地含药血清对骨髓间质干细胞(MSCs)向神经细胞分化的影响.方法 原代培养MSCs至第3代,并鉴定；熟地水煎剂分高、中、低3个剂量组对SD大鼠灌胃,10d后取大鼠血清加入培养至第三代的骨髓间质干细胞中诱导其分化,每组分别诱导24h,诱导后的细胞用免疫细胞化学法检测神经元特异性烯醇化酶(NSE)、巢蛋白(Nestin)、胶质纤维酸性蛋白(GFAP)三种蛋白.结果 原代培养鉴定为MSCs；熟地含药血清能诱导MSCs向神经细胞分化,诱导后与对照组比较,高、中、低3个剂量组细胞NSE,Nestin的表达率都较高(P＜0.05),且高、低剂量组之间比较有统计学意义(P＜0.05)；但GFAP表达极低.表明所分化的细胞为神经细胞.流式细胞仪检测结果 显示,β-巯基乙醇对细胞诱导分化能力强而促细胞增殖能力弱.结论 经典的培养方法,可分离出相对较多、纯度较高、生长状态良好的MSCs细胞；熟地含药血清能够促进MSCs向神经细胞的分化.
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| 关 键 词： | 熟地 MSCs 神经细胞 |
Effect of rehmannia drug-containing serum on mesenchymai stem cells into neural cells in vitro |
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| HUANG Yong,WU Jian-Jun,LIU Kai,et al..Effect of rehmannia drug-containing serum on mesenchymai stem cells into neural cells in vitro[J].Chinese Journal of Gerontology,2011,31(20). |
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| Authors: | HUANG Yong WU Jian-Jun LIU Kai |
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| Institution: | HUANG Yong,WU Jian-Jun,LIU Kai,et al. Gan Su College of Traditional Chinese Medicine,Lanzhou 730000,Gansu,China |
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| Abstract: | Objective To observe the effect of rehmannia drug-containing serum on bone marrow mesenchymal stem cells(BMSCs) into neural cells.Methods Primary BMSCs were cultured to the 3 rd generation and identified.SD rats were gavaged with high,medium and low-dose rehmannia decoction.10 days later,drug-containing serum was taken from rats and added to the third generation BMSCs to induce differentiation.After induction 24 hours,NSE,nestin,GFAP in cells were detected by immunocytochemistry.Results Rehmannia drug-conta... |
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| Keywords: | Rehmannia Mesenchymal stem cells Nerve cells |
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