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| 早期干预对缺氧缺血性脑损伤新生大鼠内源性神经干细胞增殖的影响 | |
| 引用本文： | 陈光福,张蕴芳,龙琦,温文,许美权,马经野. 早期干预对缺氧缺血性脑损伤新生大鼠内源性神经干细胞增殖的影响[J]. 实用儿科临床杂志, 2011, 26(20): 1575-1577,1580 |
| 作者姓名： | 陈光福 张蕴芳 龙琦 温文 许美权 马经野 |
| 作者单位： | 1. 深圳市第二人民医院深圳大学第一附属医院儿科,广东深圳,518035 2. 深圳市第二人民医院深圳大学第一附属医院病理科,广东深圳,518035 3. 深圳市第二人民医院深圳大学第一附属医院组织工程实验室,广东深圳,518035 |
| 基金项目： | 深圳市科技计划项目(200902031) |
| 摘 要： | 目的 探讨早期干预对缺氧缺血性脑损伤(HIBD)新生大鼠内源性神经干细胞(NSCs)增殖的影响.方法 健康7日龄SD新生大鼠随机分为3组:假手术对照组、HIBD组和干预组.各组又根据干预后的时间(3d、7d、14 d、28 d)随机分为4个亚组(n=10).采用Rice - Vannucci法制成HIBD模型,采用早期触摸和丰富环境刺激进行早期干预,应用免疫组织化学法检测其侧脑室外侧壁的室下带( SVZ)区BrdU、Nestin阳性细胞数.结果 干预组3d、7d、14 d和28 d组左侧SVZ区BrdU阳性细胞数[(91±12)个·mm-2、(118±17)个·mm-2、(135±24)个·mm-2、(152±31)个·mm-2]明显多于假手术对照组[(35±8)个·mm-2、(30±5)个·mm-2、(27±7)个·mm-2、(31±5)个·mm-2]和HIBD组[(62±11)个·mm-2、(59±12)个·mm-2、(34±9)个·mm-2、(36±7)个·mm-2](Pa＜0.01).干预组7d、14 d和28 d组左侧SVZ区Nestin阳性细胞数[(54±8)个·mm-2、(63±7)个·mm-2、(81±9)个·mm-2]显著高于假手术对照组[(17±3)个·mm-2、(18±2)个·mm-2、(16±3)个·mm-2]和HIBD组[(37±6)个·mm-2、(25±4)个·mm-2、(28±5)个·mm-2](Pa＜0.05,0.01).结论 早期干预可促进HIBD新生大鼠内源性NSCs的增殖. |
| 关 键 词： | 早期干预 脑损伤,缺氧缺血性 神经干细胞,内源性 大鼠 |
Effect of Early Intervention on Multiplication of Endogenous Nerve Stem Cells in Neonatal Rats with Hypoxic-Ischemic Brain Damage |
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| CHENG Guang-fu , ZHANG Yun-fang , LONG Qi , WEN Wen , XU Mei-quan , MA Jing-ye. Effect of Early Intervention on Multiplication of Endogenous Nerve Stem Cells in Neonatal Rats with Hypoxic-Ischemic Brain Damage[J]. Journal of Applied Clinical Pediatrics, 2011, 26(20): 1575-1577,1580 | |
| Authors: | CHENG Guang-fu ZHANG Yun-fang LONG Qi WEN Wen XU Mei-quan MA Jing-ye |
| Affiliation: | CHEN Guang-fu1,ZHANG Yun-fang1,LONG Qi1,WEN Wen2,XU Mei-quan2,MA Jing-ye3 (1.Department of Pediatrics,the Second People′s Hospital of Shenzhen,the First Affiliated Hospital of Shenzhen University,Shenzhen 518035,Guangdong Province,China,2.Department of Pathology,3.Tissue Engineering Laboratary,the Second People′s Hospital of Shen-zhen,the First Affiliated Hospital of Shenzhe... |
| Abstract: | Objective To explore the effect of early intervention on the multiplication of endogenous nerve stem cells(NSCs) in neonatal rats with hypoxic-ischemic brain damage(HIBD). Methods Seven-day-old Sprague-Dawley rats were randomly divided into 3 groups:the sham operation control(CON) group,the HIBD group and the intervention group.Each group were randomly divided into 3 d,7 d,14 d and 28 d subgroups according to the time after early intervention(n=10).HIBD model was prepared according to the classic Rice-Vannu... |
| Keywords: | early intervention brain injury hypoxic-ischemic nerve stem cell endogenous rat |
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