桧木醇对肾癌786-O细胞增殖及相关细胞因子的影响

目的从体外水平研究桧木醇(hinokitio1)的抗肾癌作用,探讨其作用机制。方法采用MTT法和流式细胞术检测桧木醇对肾癌786-O细胞增殖的抑制作用和促凋亡作用的影响;采用Western-blot检测桧木醇对人肾癌786-O细胞Caspase-3剪切体、LC3和P62蛋白表达水平的影响。以3-MA验证其抗癌作用与自噬作用的关系。结果桧木醇对肾癌786-O细胞增殖有显著抑制作用,主要是通过激活Caspase途径对细胞凋亡进行诱导。同时桧木醇可以对肾癌786-O细胞进行诱导自噬发生,使LC3蛋白的表达量出现显著上调,而P62蛋白表达则显著下调。结论桧木醇能够显著抑制肾癌786-O细胞的增殖,而且可以通过过度激活细胞自噬促进肾癌细胞凋亡。

Effect of Hinokitol on Proliferation of RCC 786-O and Related Cytokines

Objective To study the hinokitol anti-renal carcinoma effect in vitros ,and explore the mechanism .Methods MTT and flow cytometry assay were used to detect the effect of hinokitol on RCC 786-O cell proliferation inhibition and apoptosis;assay hinokitol influence on RCC 786-O cells caspase-3 shear body , LC3 and p62 protein expression were detected by Western blot.To verify the relationship between the anti-cancer effect and autophagy by 3-MA.Results Hinokitol can significantly inhibit the proliferation of human 786-O cells,induce cell apoptosis by activating Caspase pathway .At the same time hinokitol induced RCC 786-O cell autophagy , leading to LC3 protein expression was significantly up-regulated , while P62 protein expression was down regulated.Conclusion Hinokitol can significantly inhibit the proliferation of RCC 786-O cells,and excessive activation of autophagy can promote apoptosis of human renal carcinoma cell line .